Clinics (Oct 2018)

Association of breast cancer with human papillomavirus (HPV) infection in Northeast Brazil: molecular evidence

  • José Roosevelt Cavalcante,
  • Luiz Gonzaga Porto Pinheiro,
  • Paulo Roberto Carvalho de Almeida,
  • Márcia Valéria Pitombeira Ferreira,
  • Gizele Almada Cruz,
  • Thales Alves Campelo,
  • Caroliny Soares Silva,
  • Luana Nepomuceno Gondim Costa Lima,
  • Bruno Masato Kitagawa de Oliveira,
  • Lara Mulato Lima,
  • Laura Magda Costa Feitosa,
  • Agostinho Câmara Pinheiro,
  • Cristiane Cunha Frota

DOI
https://doi.org/10.6061/clinics/2018/e465
Journal volume & issue
Vol. 73, no. 0

Abstract

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OBJECTIVE: The aim of this study is to investigate the presence of human papillomavirus DNA and genotypes in breast cancer and normal breast tissue samples obtained from women from the northeast region of Brazil. METHOD: One hundred three breast cancer samples and 95 normal breast samples, as the non-malignant controls, were studied. DNA extraction was verified by human beta-globin gene amplification, and polymerase chain reaction was conducted based on HPV L1-specific consensus primers MY09/MY11 and GP5+/GP6+, followed by nested multiplex polymerase chain reaction with type-specific primers for the E6/E7 consensus region. RESULTS: Human papillomavirus DNA was detected in 51 (49.5%) breast carcinoma samples and 15 (15.8%) normal breast samples (p<0.0001). Human papillomavirus genotypes 6 and 11 were identified in 15.2% of all samples. CONCLUSIONS: The high frequency of human papillomavirus infection in breast cancer samples indicates a potential role of this virus in breast carcinogenesis in the studied participants.

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