Comparison of the Pathogenicity in Mice of A(H1N1)pdm09 Viruses Isolated between 2009 and 2015 in Japan

Hiromichi Mitake; Atsuhiro Yasuhara; Tiago  J. S. Lopes; Yuko Tagawa-Sakai; Kohei Shimizu; Hiroki Ozawa; Chiharu Kawakami; Saeko Morikawa; Norio Sugaya; Tokiko Watanabe; Yoshihiro Kawaoka

doi:10.3390/v12020155

Viruses (Jan 2020)

Comparison of the Pathogenicity in Mice of A(H1N1)pdm09 Viruses Isolated between 2009 and 2015 in Japan

Hiromichi Mitake,
Atsuhiro Yasuhara,
Tiago J. S. Lopes,
Yuko Tagawa-Sakai,
Kohei Shimizu,
Hiroki Ozawa,
Chiharu Kawakami,
Saeko Morikawa,
Norio Sugaya,
Tokiko Watanabe,
Yoshihiro Kawaoka

Affiliations

Hiromichi Mitake: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan
Atsuhiro Yasuhara: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan
Tiago J. S. Lopes: Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53711, USA
Yuko Tagawa-Sakai: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan
Kohei Shimizu: Yokohama City Institute of Public Health, Kanagawa 236-0051, Japan
Hiroki Ozawa: Yokohama City Institute of Public Health, Kanagawa 236-0051, Japan
Chiharu Kawakami: Yokohama City Institute of Public Health, Kanagawa 236-0051, Japan
Saeko Morikawa: Division of Virology, Department of Microbiology, Osaka Institute of Public Health, Osaka 537-0025, Japan
Norio Sugaya: Department of Infection Control and Pediatrics, Keiyu Hospital, Yokohama 220-0012, Japan
Tokiko Watanabe: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan
Yoshihiro Kawaoka: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan

DOI: https://doi.org/10.3390/v12020155
Journal volume & issue: Vol. 12, no. 2
p. 155

Abstract

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The A(H1N1)pdm09 virus emerged in 2009 and continues to circulate in human populations. Recent A(H1N1)pdm09 viruses, that is, A(H1N1)pdm09 viruses circulating in the post-pandemic era, can cause more or less severe infections than those caused by the initial pandemic viruses. To evaluate the changes in pathogenicity of the A(H1N1)pdm09 viruses during their continued circulation in humans, we compared the nucleotide and amino acid sequences of ten A(H1N1)pdm09 viruses isolated in Japan between 2009 and 2015, and experimentally infected mice with each virus. The severity of infection caused by these Japanese isolates ranged from milder to more severe than that caused by the prototypic pandemic strain A/California/04/2009 (CA04/09); however, specific mutations responsible for their pathogenicity have not yet been identified.

Published in Viruses

ISSN: 1999-4915 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.mdpi.com/journal/viruses

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