Analysis of Cell Motility with a Successive Sequence of Images

Abstract

Analysis of cell motility usually requires several hours of continuousvideo recording of a single view field. Consequently, the method is very much timeconsuming and only a small number of cells is usually included into analysis. To increase the number of cells monitored in an experiment we developed a simpleand expeditious method for analysing the cell motility. A combination of successive series of images of individual cells relocated on CELLocate coverslips andvector graphic editing software Adobe Illustrator 9.0 enabled simultaneous monitoring of locomotion for up to ten times more cells as with previous methods.

Keywords

• Cell motility
• digital camera
• successive sequence of images
• software Adobe Illustrator 9.0