Morphological and Molecular Characterization of <i>Discolaimus haridwarensis</i> sp. n. (Nematoda: Dorylaimida: Qudsianematidae) from India

Abstract

Discolaimus haridwarensis sp. n. is described from agricultural fields of sugarcane in the Haridwar district, India. It is characterized by its 2.11–2.32 mm long body, lip region offset by deep constriction and 27–30 μm wide, odontostyle 20–23 μm long with aperture occupying 50–54% of its length, 436–487 μm long neck, pharyngeal expansion 57–59% of total neck length, uterus a simple tube-like structure 22–31 μm long or 0.3–0.5 times the corresponding body diameter, pars refringens vaginae absent, transverse vulva (V = 48–55), female tail conoid (34–40 µm, c = 53–68, c′ = 1.0–1.3) with rounded terminus, and males absent. The phylogenetic analysis inferred from the D2-D3 expansion segments of 28S rRNA gene and 18S rRNA gene sequences showed that Discolaimus haridwarensis sp. n. clustered with other dorylaimid species from the genus Discolaimus and the subfamily Discolaiminae.

Keywords

• Bayesian inference
• D2-D3 expansion segments
• description
• SSU and LSU ribosomal DNA
• morphology
• morphometrics