A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds

Frederic Strobl; Ernst H. K. Stelzer

doi:10.1038/s41598-021-94288-0

Scientific Reports (Jul 2021)

A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds

Frederic Strobl,
Ernst H. K. Stelzer

Affiliations

Frederic Strobl: Physical Biology/Physikalische Biologie (IZN, FB 15), Buchmann Institute for Molecular Life Sciences (BMLS), Cluster of Excellence Frankfurt – Macromolecular Complexes (CEF – MC), Goethe-Universität Frankfurt Am Main (Campus Riedberg)
Ernst H. K. Stelzer: Physical Biology/Physikalische Biologie (IZN, FB 15), Buchmann Institute for Molecular Life Sciences (BMLS), Cluster of Excellence Frankfurt – Macromolecular Complexes (CEF – MC), Goethe-Universität Frankfurt Am Main (Campus Riedberg)

DOI: https://doi.org/10.1038/s41598-021-94288-0
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 9

Abstract

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Abstract We present a deterministic workflow for genotyping single and double transgenic individuals directly upon nascence that prevents overproduction and reduces wasted animals by two-thirds. In our vector concepts, transgenes are accompanied by two of four clearly distinguishable transformation markers that are embedded in interweaved, but incompatible Lox site pairs. Following Cre-mediated recombination, the genotypes of single and double transgenic individuals were successfully identified by specific marker combinations in 461 scorings.

Published in Scientific Reports

ISSN: 2045-2322 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Medicine; Science
Website: https://www.nature.com/srep/

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