Generation of a homozygous ARHGAP11B knockout hiPSC line by CRISPR/Cas9 system

Yu Liu; Yinge Jin; Tao Chen; Yunqin Wu; Xiaohua Peng; Wansheng Li; Shu Wei; Min Chen; Qingjian Zou; Suqin Guo; Jucai Xu; Chengcheng Tang; Xiaoqing Zhou

Stem Cell Research (May 2022)

Generation of a homozygous ARHGAP11B knockout hiPSC line by CRISPR/Cas9 system

Yu Liu,
Yinge Jin,
Tao Chen,
Yunqin Wu,
Xiaohua Peng,
Wansheng Li,
Shu Wei,
Min Chen,
Qingjian Zou,
Suqin Guo,
Jucai Xu,
Chengcheng Tang,
Xiaoqing Zhou

Affiliations

Yu Liu: Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Biotechnology and Health Science, International Healthcare Innovation Institute, Wuyi University, Jiangmen 529020, China
Yinge Jin: Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Biotechnology and Health Science, International Healthcare Innovation Institute, Wuyi University, Jiangmen 529020, China
Tao Chen: Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Biotechnology and Health Science, International Healthcare Innovation Institute, Wuyi University, Jiangmen 529020, China
Yunqin Wu: Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Biotechnology and Health Science, International Healthcare Innovation Institute, Wuyi University, Jiangmen 529020, China
Xiaohua Peng: Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Biotechnology and Health Science, International Healthcare Innovation Institute, Wuyi University, Jiangmen 529020, China
Wansheng Li: Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Biotechnology and Health Science, International Healthcare Innovation Institute, Wuyi University, Jiangmen 529020, China
Shu Wei: State Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan, Center for Life Science, School of Life Sciences, Yunnan University, Kunming 650504, China
Min Chen: Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Biotechnology and Health Science, International Healthcare Innovation Institute, Wuyi University, Jiangmen 529020, China
Qingjian Zou: Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Biotechnology and Health Science, International Healthcare Innovation Institute, Wuyi University, Jiangmen 529020, China
Suqin Guo: Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Biotechnology and Health Science, International Healthcare Innovation Institute, Wuyi University, Jiangmen 529020, China
Jucai Xu: Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Biotechnology and Health Science, International Healthcare Innovation Institute, Wuyi University, Jiangmen 529020, China
Chengcheng Tang: Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Biotechnology and Health Science, International Healthcare Innovation Institute, Wuyi University, Jiangmen 529020, China; Corresponding authors.
Xiaoqing Zhou: Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Biotechnology and Health Science, International Healthcare Innovation Institute, Wuyi University, Jiangmen 529020, China; Corresponding authors.

Journal volume & issue: Vol. 61
p. 102764

Abstract

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The human specific gene ARHGAP11B is preferentially expressed in neural progenitors of fetal neocortex and plays a key role in the evolutionary expansion of the neocortex. Here, we generated a homozygous ARHGAP11B knockout human induced pluripotent stem cell (hiPSC) line through CRISPR/Cas9 gene editing system. ARHGAP11B deficient cell line maintained a normal karyotype (46, XX), expressed pluripotency markers, and showed the capability to spontaneously differentiate into all three germ layers in vivo. The ARHGAP11B knockout cell line can provide a new cell model for studying the evolution of human neocortex.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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