RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency

Jun Song; Dongshan Yang; Jie Xu; Tianqing Zhu; Y. Eugene Chen; Jifeng Zhang

doi:10.1038/ncomms10548

Nature Communications (Jan 2016)

RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency

Jun Song,
Dongshan Yang,
Jie Xu,
Tianqing Zhu,
Y. Eugene Chen,
Jifeng Zhang

Affiliations

Jun Song: Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center
Dongshan Yang: Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center
Jie Xu: Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center
Tianqing Zhu: Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center
Y. Eugene Chen: Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center
Jifeng Zhang: Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center

DOI: https://doi.org/10.1038/ncomms10548
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 7

Abstract

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CRISPR/Cas9 and transcription activator-like effector nuclease (TALEN) are becoming major tools for genome editing. Here, Song et al. show that RS-1, a small-molecule enhancer for homology directed repair, increases the CRISPR/Cas9 and TALEN mediated knock-in efficiency both in vitro and in vivowith rabbit.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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