Rad52 competes with Ku70/Ku86 for binding to S-region DSB ends to modulate antibody class-switch DNA recombination

Hong Zan; Connie Tat; Zhifang Qiu; Julia R. Taylor; Justin A. Guerrero; Tian Shen; Paolo Casali

doi:10.1038/ncomms14244

Nature Communications (Feb 2017)

Rad52 competes with Ku70/Ku86 for binding to S-region DSB ends to modulate antibody class-switch DNA recombination

Hong Zan,
Connie Tat,
Zhifang Qiu,
Julia R. Taylor,
Justin A. Guerrero,
Tian Shen,
Paolo Casali

Affiliations

Hong Zan: Department of Microbiology, Immunology and Molecular Genetics, University of Texas School of Medicine, UT Health Science Center
Connie Tat: Department of Microbiology, Immunology and Molecular Genetics, University of Texas School of Medicine, UT Health Science Center
Zhifang Qiu: Department of Microbiology, Immunology and Molecular Genetics, University of Texas School of Medicine, UT Health Science Center
Julia R. Taylor: Department of Microbiology, Immunology and Molecular Genetics, University of Texas School of Medicine, UT Health Science Center
Justin A. Guerrero: Department of Microbiology, Immunology and Molecular Genetics, University of Texas School of Medicine, UT Health Science Center
Tian Shen: Department of Microbiology, Immunology and Molecular Genetics, University of Texas School of Medicine, UT Health Science Center
Paolo Casali: Department of Microbiology, Immunology and Molecular Genetics, University of Texas School of Medicine, UT Health Science Center

DOI: https://doi.org/10.1038/ncomms14244
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 16

Abstract

Read online

Class switch DNA recombination (CSR) is critical for maturation of antibody response, and relies on Ku-mediated NHEJ of DSBs in the IgH S regions for recombination. This study shows Rad52 contributes to CSR through a Ku-independent alternative NHEJ that introduces microhomologies in S–S junctions.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal