Role and mechanism of NTR p75 receptor in oxidative damage of retinal pigment epithelium cells

Abstract

AIM: To study the oxidative damage and mechanism of p75 NTR receptor in retinal pigment epithelial cells(RPE). METHODS: The NTR p75 receptor was used to transfer the retinal pigment epithelium cells as the experimental group, and the non transfected retinal pigment epithelium cells were used as control group. The BrdU test detected the proliferation of two groups of cells. The rate of apoptosis in two sets of apoptosis was measured by PI/in the V-FITC double dye method. The laser microscope detects the ROS levels within the cell. The flow cytometer detected the levels of ROS, mitochondrial markers, cytochrome C expression in RPE cells. The Western blot method detected the expression level of Fas, Caspase-3, and VEGF165 in RPE cells. RESULTS: The RPE cell proliferation activity was gradually decreasing(PPPPPPPCONCLUSION: The over expression of p75 NTR receptor could lead to damage of mitochondria in retinal pigment epithelium cells, but it could also promote the apoptosis reaction, eventually it led to the formation of choroidal neovascularization, so it could be speculated that p75 NTR receptor is the damage factors of retinal pigment epithelium.

Keywords

• retinal pigment epithelial cells
• NTR p75 receptor
• choroidal neovascularization
• injury
• injury