Frontiers in Microbiology (May 2022)
Performance of SARS-CoV-2 Antigen-Detecting Rapid Diagnostic Tests for Omicron and Other Variants of Concern
- Dàlia Raïch-Regué,
- Jordana Muñoz-Basagoiti,
- Daniel Perez-Zsolt,
- Marc Noguera-Julian,
- Marc Noguera-Julian,
- Marc Noguera-Julian,
- Edwards Pradenas,
- Eva Riveira-Muñoz,
- Neus Giménez,
- Assumpta Carabaza,
- Francesc Giménez,
- Verónica Saludes,
- Verónica Saludes,
- Elisa Martró,
- Elisa Martró,
- Neus Robert,
- Ignacio Blanco,
- Roger Paredes,
- Roger Paredes,
- Lidia Ruiz,
- Ester Ballana,
- Bonaventura Clotet,
- Bonaventura Clotet,
- Bonaventura Clotet,
- Julià Blanco,
- Julià Blanco,
- Julià Blanco,
- Nuria Izquierdo-Useros,
- Nuria Izquierdo-Useros
Affiliations
- Dàlia Raïch-Regué
- IrsiCaixa AIDS Research Institute, Germans Trias i Pujol Research Institute (IGTP), Badalona, Spain
- Jordana Muñoz-Basagoiti
- IrsiCaixa AIDS Research Institute, Germans Trias i Pujol Research Institute (IGTP), Badalona, Spain
- Daniel Perez-Zsolt
- IrsiCaixa AIDS Research Institute, Germans Trias i Pujol Research Institute (IGTP), Badalona, Spain
- Marc Noguera-Julian
- IrsiCaixa AIDS Research Institute, Germans Trias i Pujol Research Institute (IGTP), Badalona, Spain
- Marc Noguera-Julian
- AIDS and Infectious Deseases Department, University of Vic–Central University of Catalonia (UVic-UCC), Vic, Spain
- Marc Noguera-Julian
- Infectious Disease Networking Biomedical Research Center, Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), Carlos III Health Institute, Madrid, Spain
- Edwards Pradenas
- IrsiCaixa AIDS Research Institute, Germans Trias i Pujol Research Institute (IGTP), Badalona, Spain
- Eva Riveira-Muñoz
- IrsiCaixa AIDS Research Institute, Germans Trias i Pujol Research Institute (IGTP), Badalona, Spain
- Neus Giménez
- Nesapor Europa, Premià de Dalt, Spain
- Assumpta Carabaza
- Nesapor Europa, Premià de Dalt, Spain
- Francesc Giménez
- Nesapor Europa, Premià de Dalt, Spain
- Verónica Saludes
- Laboratori Clínic Metropolitana Nord, Department of Microbiology, Germans Trias i Pujol University Hospital and Research Institute (IGTP), Badalona, Spain
- Verónica Saludes
- Epidemiology and Public Health Networking Biomedical Research Centre (CIBERESP), Instituto de Salud Carlos III, Madrid, Spain
- Elisa Martró
- Laboratori Clínic Metropolitana Nord, Department of Microbiology, Germans Trias i Pujol University Hospital and Research Institute (IGTP), Badalona, Spain
- Elisa Martró
- Epidemiology and Public Health Networking Biomedical Research Centre (CIBERESP), Instituto de Salud Carlos III, Madrid, Spain
- Neus Robert
- Department of Emergency, Germans Trias i Pujol University Hospital and Research Institute (IGTP), Badalona, Spain
- Ignacio Blanco
- Laboratori Clínic Metropolitana Nord, Department of Microbiology, Germans Trias i Pujol University Hospital and Research Institute (IGTP), Badalona, Spain
- Roger Paredes
- IrsiCaixa AIDS Research Institute, Germans Trias i Pujol Research Institute (IGTP), Badalona, Spain
- Roger Paredes
- Infectious Disease Networking Biomedical Research Center, Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), Carlos III Health Institute, Madrid, Spain
- Lidia Ruiz
- IrsiCaixa AIDS Research Institute, Germans Trias i Pujol Research Institute (IGTP), Badalona, Spain
- Ester Ballana
- IrsiCaixa AIDS Research Institute, Germans Trias i Pujol Research Institute (IGTP), Badalona, Spain
- Bonaventura Clotet
- IrsiCaixa AIDS Research Institute, Germans Trias i Pujol Research Institute (IGTP), Badalona, Spain
- Bonaventura Clotet
- AIDS and Infectious Deseases Department, University of Vic–Central University of Catalonia (UVic-UCC), Vic, Spain
- Bonaventura Clotet
- Infectious Disease Networking Biomedical Research Center, Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), Carlos III Health Institute, Madrid, Spain
- Julià Blanco
- IrsiCaixa AIDS Research Institute, Germans Trias i Pujol Research Institute (IGTP), Badalona, Spain
- Julià Blanco
- AIDS and Infectious Deseases Department, University of Vic–Central University of Catalonia (UVic-UCC), Vic, Spain
- Julià Blanco
- Infectious Disease Networking Biomedical Research Center, Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), Carlos III Health Institute, Madrid, Spain
- Nuria Izquierdo-Useros
- IrsiCaixa AIDS Research Institute, Germans Trias i Pujol Research Institute (IGTP), Badalona, Spain
- Nuria Izquierdo-Useros
- Infectious Disease Networking Biomedical Research Center, Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), Carlos III Health Institute, Madrid, Spain
- DOI
- https://doi.org/10.3389/fmicb.2022.810576
- Journal volume & issue
-
Vol. 13
Abstract
The SARS-CoV-2 antigen-detecting rapid diagnostic test (Ag-RDTs) is an easy-to-use diagnostic tool to identify the contagious individuals and reduce the new infections. However, to be effective, Ag-RDTs require the detection of distinct variants of concern (VOC) with high analytical sensitivity. Here, we found that the VOC diverge at the nucleocapsid protein used by four commercial Ag-RDTs for the viral detection. Relative to the original D614G variant, there was a 10-fold loss of detection for the Delta and Alpha variants in certain Ag-RDTs, a reduction above the threshold required to isolate the viable virus. However, Beta and Omicron variants did not lose the detection capacity. As the new VOC arise, successful contact tracing requires continuous monitoring of Ag-RDTs performance.
Keywords
