Establishment of a non-integrated iPS cell line (SDQLCHi072-A) from a patient suffered from AUTS2 syndrome

Zilong Li; Zhengbin Li; Yuqiang Lv; Min Gao; Ning Liu; Zhongtao Gai; Qiji Liu

Stem Cell Research (Dec 2024)

Establishment of a non-integrated iPS cell line (SDQLCHi072-A) from a patient suffered from AUTS2 syndrome

Zilong Li,
Zhengbin Li,
Yuqiang Lv,
Min Gao,
Ning Liu,
Zhongtao Gai,
Qiji Liu

Affiliations

Zilong Li: Key Laboratory for Experimental Teratology of the Ministry of Education and Department of Medical Genetics, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, China; Shandong Liabo Biotechnology Co., Ltd., Jinan, Shandong 250100, China; Pediatric Research Institute, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China; Pediatric Research Institute, Jinan Children’s Hospital, Jinan, Shandong 250022, China
Zhengbin Li: Key Laboratory for Experimental Teratology of the Ministry of Education and Department of Medical Genetics, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, China; Pediatric Research Institute, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China; Pediatric Research Institute, Jinan Children’s Hospital, Jinan, Shandong 250022, China
Yuqiang Lv: Pediatric Research Institute, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China; Pediatric Research Institute, Jinan Children’s Hospital, Jinan, Shandong 250022, China
Min Gao: Pediatric Research Institute, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China; Pediatric Research Institute, Jinan Children’s Hospital, Jinan, Shandong 250022, China
Ning Liu: Pediatric Research Institute, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China; Pediatric Research Institute, Jinan Children’s Hospital, Jinan, Shandong 250022, China
Zhongtao Gai: Shandong Liabo Biotechnology Co., Ltd., Jinan, Shandong 250100, China; Pediatric Research Institute, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China; Pediatric Research Institute, Jinan Children’s Hospital, Jinan, Shandong 250022, China; Corresponding authors.
Qiji Liu: Key Laboratory for Experimental Teratology of the Ministry of Education and Department of Medical Genetics, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, China; Corresponding authors.

Journal volume & issue: Vol. 81
p. 103554

Abstract

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In this study, PBMCs used in reprogramming were from a boy suffered from AUTS2 syndrome confirmed by clinical and genetic diagnosis. iPSCs were established by non-integrated method, which carried AUTS2 heterozygous mutation. The established iPSCs presented similar appearance and expressed pluripotent markers in mRNA and protein level. Additionally, the iPSCs possessed favorable differentiative capacity in vivo and normal karyotype. This is an effective potential model for the study about AUTS2 syndrome and the screen about drug therapy.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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