Mitotic activity, submicroscopic organization and respiration of meristematic cells of root tips of Allium cepa L. treated with dacarbazine (DTIC)

Abstract

Studies were carried out on the effect of dacarbazine (DTIC) on meristematic cells of root tips of Allium cepa L. Noticeable mitodepression was observed. It depended upon time of exposure and DTIC concentration. Mitodepression was caused by an inhibition of DNA synthesis and blocking of the G2 phase. DTIC caused also disturbances of the karyokinesis and cytokinesis, similar as in case of colchicine effect. In the experimental conditions inhibition of cell respiration was noted, as also changes in the tissue ultrastructure. These changes were typical of the effect of respiratory inhibitors.