A method for high-throughput PCR-based genotyping of larval zebrafish tail biopsies

Abstract

Here we describe a method for high-throughput genotyping of live larval zebrafish as early as 72 h post-fertilization (hpf). Importantly, this technique allows rapid and cost-effective PCR-based genotyping from very small fin biopsies, which regenerate as the embryo develops, thereby allowing researchers to select embryos with desired genotypes to be raised to adulthood.

Keywords

• zebrafish
• embryo
• genotyping
• PCR
• fin clip