Reduction of GTP Cyclohydrolase I Feedback Regulating Protein Expression by Hydrogen Peroxide in Vascular Endothelial Cells

Masakazu Ishii; Shunichi Shimizu; Teruaki Wajima; Tamio Hagiwara; Takaharu Negoro; Akira Miyazaki; Takashi Tobe; Yuji Kiuchi

Journal of Pharmacological Sciences (Jan 2005)

Reduction of GTP Cyclohydrolase I Feedback Regulating Protein Expression by Hydrogen Peroxide in Vascular Endothelial Cells

Masakazu Ishii,
Shunichi Shimizu,
Teruaki Wajima,
Tamio Hagiwara,
Takaharu Negoro,
Akira Miyazaki,
Takashi Tobe,
Yuji Kiuchi

Affiliations

Masakazu Ishii: Departments of Pathophysiology, Showa University, Shinagawa-ku, Tokyo 142-8555, Japan; Corresponding author. FAX: +81-3-3786-0481 E-mail: [email protected]
Shunichi Shimizu: Departments of Pathophysiology, Showa University, Shinagawa-ku, Tokyo 142-8555, Japan
Teruaki Wajima: Departments of Pathophysiology, Showa University, Shinagawa-ku, Tokyo 142-8555, Japan
Tamio Hagiwara: Department of Biochemistry, School of Medicine, Showa University, Shinagawa-ku, Tokyo 142-8555, Japan
Takaharu Negoro: Departments of Pathophysiology, Medicinal Information, School of Pharmaceutical Sciences, Showa University, Shinagawa-ku, Tokyo 142-8555, Japan
Akira Miyazaki: Department of Biochemistry, School of Medicine, Showa University, Shinagawa-ku, Tokyo 142-8555, Japan
Takashi Tobe: Departments of Pathophysiology, Medicinal Information, School of Pharmaceutical Sciences, Showa University, Shinagawa-ku, Tokyo 142-8555, Japan
Yuji Kiuchi: Departments of Pathophysiology, Showa University, Shinagawa-ku, Tokyo 142-8555, Japan

Journal volume & issue: Vol. 97, no. 2
pp. 299 – 302

Abstract

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We examined the effect of H2O2 on the expression of GTP cyclohydrolase I (GTPCH) feedback regulating protein (GFRP). Addition of H2O2 to endothelial cells decreased GFRP mRNA levels, in contrast to the increase of tetrahydrobiopterin (BH4) content and GTPCH mRNA levels. The inhibitors of nitric oxide (NO) synthase and GTPCH had no influence on the decrease of GFRP mRNA levels in H2O2-treated cells. It is suggested that H2O2 induces BH4 synthesis through not only induction of GTPCH but also reduction of GFRP. The decrease of GFRP mRNA level appears to be independent of the produced NO and BH4. Keywords:: GTP cyclohydrolase I, GTP cyclohydrolase I feedback regulating protein, hydrogen peroxide

Published in Journal of Pharmacological Sciences

ISSN: 1347-8613 (Print)
Publisher: Elsevier
Country of publisher: Japan
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://www.journals.elsevier.com/journal-of-pharmacological-sciences

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