Surface Modification of Biomaterials for Conjugation with Human Fetal Osteoblasts

Abstract

Surface functionalization of hydroxyapatite (HA) and ?-tricalcium phosphate (TCP) bioceramics with chemical ligands containing a pyrrogallol moiety was developed to improve the adhesion of bone cell precursors to the biomaterials. Fast and biocompatible copper-free click reaction with azido-modified human fetal osteoblasts resulted in improved cell binding to both HA and TCP bioceramics, opening the way for using this methodology in the preparation of cell-engineered bone implants.

Keywords

• Cell adhesions
• Copper-free click reaction
• Functionalized biomaterials
• Human fetal osteoblasts