Exendin-4 promotes actin cytoskeleton rearrangement and protects cells from Nogo-A-Δ20 mediated spreading inhibition and growth cone collapse by down-regulating RhoA expression and activation via the PI3K pathway

Fei Zhao; jianwei Li; Renjie Wang; Huiyou Xu; Ke Ma; Xianbin Kong; Zhonglei Sun; Xuegang Niu; Jipeng Jiang; Baohu Liu; Bo Li; Feng Duan; Xuyi Chen

Biomedicine & Pharmacotherapy (Jan 2019)

Exendin-4 promotes actin cytoskeleton rearrangement and protects cells from Nogo-A-Δ20 mediated spreading inhibition and growth cone collapse by down-regulating RhoA expression and activation via the PI3K pathway

Fei Zhao,
jianwei Li,
Renjie Wang,
Huiyou Xu,
Ke Ma,
Xianbin Kong,
Zhonglei Sun,
Xuegang Niu,
Jipeng Jiang,
Baohu Liu,
Bo Li,
Feng Duan,
Xuyi Chen

Affiliations

Fei Zhao: Affiliated Hospital of the Logistics University of the Chinese People’s Armed Police Force and Jinzhou Medical University Cooperative Training Center, Tianjin, 300162, China; Jinzhou Medical University, Jinzhou, Liaoning Province, 121001, China; Tianjin Key Laboratory of Neurotrauma Repair, Institute of Traumatic Brain Injury and Neuroscience, Center for Neurology and Neurosurgery, Affiliated Hospital of The Logistics University of the Chinese People’s Armed Police Force, Tianjin, 300162, China
jianwei Li: Tianjin Key Laboratory of Neurotrauma Repair, Institute of Traumatic Brain Injury and Neuroscience, Center for Neurology and Neurosurgery, Affiliated Hospital of The Logistics University of the Chinese People’s Armed Police Force, Tianjin, 300162, China
Renjie Wang: Tianjin Key Laboratory of Neurotrauma Repair, Institute of Traumatic Brain Injury and Neuroscience, Center for Neurology and Neurosurgery, Affiliated Hospital of The Logistics University of the Chinese People’s Armed Police Force, Tianjin, 300162, China
Huiyou Xu: Tianjin Key Laboratory of Neurotrauma Repair, Institute of Traumatic Brain Injury and Neuroscience, Center for Neurology and Neurosurgery, Affiliated Hospital of The Logistics University of the Chinese People’s Armed Police Force, Tianjin, 300162, China
Ke Ma: Tianjin Key Laboratory of Neurotrauma Repair, Institute of Traumatic Brain Injury and Neuroscience, Center for Neurology and Neurosurgery, Affiliated Hospital of The Logistics University of the Chinese People’s Armed Police Force, Tianjin, 300162, China
Xianbin Kong: Tianjin Key Laboratory of Neurotrauma Repair, Institute of Traumatic Brain Injury and Neuroscience, Center for Neurology and Neurosurgery, Affiliated Hospital of The Logistics University of the Chinese People’s Armed Police Force, Tianjin, 300162, China; Graduate school of Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China
Zhonglei Sun: Affiliated Hospital of the Logistics University of the Chinese People’s Armed Police Force and Jinzhou Medical University Cooperative Training Center, Tianjin, 300162, China; Jinzhou Medical University, Jinzhou, Liaoning Province, 121001, China; Tianjin Key Laboratory of Neurotrauma Repair, Institute of Traumatic Brain Injury and Neuroscience, Center for Neurology and Neurosurgery, Affiliated Hospital of The Logistics University of the Chinese People’s Armed Police Force, Tianjin, 300162, China
Xuegang Niu: Affiliated Hospital of the Logistics University of the Chinese People’s Armed Police Force and Jinzhou Medical University Cooperative Training Center, Tianjin, 300162, China; Jinzhou Medical University, Jinzhou, Liaoning Province, 121001, China; Tianjin Fourth Central Hospital, Tianjin, 300140, China
Jipeng Jiang: Tianjin Key Laboratory of Neurotrauma Repair, Institute of Traumatic Brain Injury and Neuroscience, Center for Neurology and Neurosurgery, Affiliated Hospital of The Logistics University of the Chinese People’s Armed Police Force, Tianjin, 300162, China
Baohu Liu: Tianjin Key Laboratory of Neurotrauma Repair, Institute of Traumatic Brain Injury and Neuroscience, Center for Neurology and Neurosurgery, Affiliated Hospital of The Logistics University of the Chinese People’s Armed Police Force, Tianjin, 300162, China; Wang Jing Hospital of China Academy of Chinese Medical Sciences, Beijing, 100102, China
Bo Li: Tianjin Key Laboratory of Neurotrauma Repair, Institute of Traumatic Brain Injury and Neuroscience, Center for Neurology and Neurosurgery, Affiliated Hospital of The Logistics University of the Chinese People’s Armed Police Force, Tianjin, 300162, China
Feng Duan: College of Computer and Control Engineering, Nankai University, Tianjin, 300071, China; Corresponding author.
Xuyi Chen: Tianjin Key Laboratory of Neurotrauma Repair, Institute of Traumatic Brain Injury and Neuroscience, Center for Neurology and Neurosurgery, Affiliated Hospital of The Logistics University of the Chinese People’s Armed Police Force, Tianjin, 300162, China; Corresponding author.

Journal volume & issue: Vol. 109
pp. 135 – 143

Abstract

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Exendin-4 is a protein of the GLP-1 family currently used to treat diabetes. Recently, a greater number of biological properties have been associated with the GLP-1 family. Our data shows that exendin-4 treatment significantly increases the cytoskeleton rearrangement, which leads to an increasingly differentiated phenotype and reduced cell migration. We also found that exendin-4 could prevent SH-SY5Y and PC12 cells from Nogo-A-Δ20 mediated spreading inhibition and neurite collapse. Western blot analysis indicated that exendin-4 treatment both reduced the expression and activation of RhoA via the PI3K signaling pathway. These data suggest that exendin-4 may protect nerve regeneration by preventing the inhibition of Nogo-A via down-regulating RhoA expression and activation.

Published in Biomedicine & Pharmacotherapy

ISSN: 0753-3322 (Print); 1950-6007 (Online)
Publisher: Elsevier
Country of publisher: France
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: https://www.journals.elsevier.com/biomedicine-and-pharmacotherapy

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