DNA nicks induce mutational signatures associated with BRCA1 deficiency

Yi-Li Feng; Qian Liu; Ruo-Dan Chen; Si-Cheng Liu; Zhi-Cheng Huang; Kun-Ming Liu; Xiao-Ying Yang; An-Yong Xie

doi:10.1038/s41467-022-32011-x

Nature Communications (Jul 2022)

DNA nicks induce mutational signatures associated with BRCA1 deficiency

Yi-Li Feng,
Qian Liu,
Ruo-Dan Chen,
Si-Cheng Liu,
Zhi-Cheng Huang,
Kun-Ming Liu,
Xiao-Ying Yang,
An-Yong Xie

Affiliations

Yi-Li Feng: Innovation Center for Minimally Invasive Technique and Device, Department of General Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine
Qian Liu: Innovation Center for Minimally Invasive Technique and Device, Department of General Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine
Ruo-Dan Chen: Innovation Center for Minimally Invasive Technique and Device, Department of General Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine
Si-Cheng Liu: Innovation Center for Minimally Invasive Technique and Device, Department of General Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine
Zhi-Cheng Huang: Innovation Center for Minimally Invasive Technique and Device, Department of General Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine
Kun-Ming Liu: Innovation Center for Minimally Invasive Technique and Device, Department of General Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine
Xiao-Ying Yang: Innovation Center for Minimally Invasive Technique and Device, Department of General Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine
An-Yong Xie: Innovation Center for Minimally Invasive Technique and Device, Department of General Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine

DOI: https://doi.org/10.1038/s41467-022-32011-x
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 15

Abstract

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It remains unclear how BRCA1-associated mutational sigantures are generated in cancer. Here, the authors develop nCas9-based approaches to uncover that aberrant repair of one-ended DSBs converted from DNA nicks by replication, not that of two-ended DSBs, induces such mutational signatures.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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