Assessment of neurovascular dynamics during transient ischemic attack by the novel integration of micro-electrocorticography electrode array with functional photoacoustic microscopy

Yu-Hang Liu; Lun-De Liao; Stacey Sze Hui Tan; Ki Yong Kwon; Ji Min Ling; Aishwarya Bandla; Yen-Yu Ian Shih; Eddie Tung Wee Tan; Wen Li; Wai Hoe Ng; Hsin-Yi Lai; You-Yin Chen; Nitish V. Thakor

Neurobiology of Disease (Oct 2015)

Assessment of neurovascular dynamics during transient ischemic attack by the novel integration of micro-electrocorticography electrode array with functional photoacoustic microscopy

Yu-Hang Liu,
Lun-De Liao,
Stacey Sze Hui Tan,
Ki Yong Kwon,
Ji Min Ling,
Aishwarya Bandla,
Yen-Yu Ian Shih,
Eddie Tung Wee Tan,
Wen Li,
Wai Hoe Ng,
Hsin-Yi Lai,
You-Yin Chen,
Nitish V. Thakor

Affiliations

Yu-Hang Liu: Singapore Institute for Neurotechnology (SINAPSE), National University of Singapore, 28 Medical Drive, #05-COR, 117456, Singapore; Department of Electrical and Computer Engineering, National University of Singapore, 21 Lower Kent Ridge Rd, 119077, Singapore
Lun-De Liao: Singapore Institute for Neurotechnology (SINAPSE), National University of Singapore, 28 Medical Drive, #05-COR, 117456, Singapore; Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, 35 Keyan Road., Zhunan, Miaoli, Taiwan; Corresponding author at: Singapore Institute for Neurotechnology (SINAPSE), National University of Singapore, Life Sciences Institute, 28 Medical Drive, #05-COR, 117456 Singapore, and Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, 35 Keyan Road., Zhunan, Miaoli, Taiwan.
Stacey Sze Hui Tan: Singapore Institute for Neurotechnology (SINAPSE), National University of Singapore, 28 Medical Drive, #05-COR, 117456, Singapore
Ki Yong Kwon: Department of Electrical and Computer Engineering, Michigan State University, 428 S Shaw Ln, RM 2120 Engineering Building, MI 48824, USA
Ji Min Ling: Singapore Institute for Neurotechnology (SINAPSE), National University of Singapore, 28 Medical Drive, #05-COR, 117456, Singapore; Department of Neurosurgery, National Neuroscience Institute (NNI), 11 Jalan Tan Tock Seng, 308433, Singapore; SingHealth Duke-NUS Neuroscience Academic Clinical Program, National Neuroscience Institute (NNI), 11 Jalan Tan Tock Seng, 308433 Singapore
Aishwarya Bandla: Singapore Institute for Neurotechnology (SINAPSE), National University of Singapore, 28 Medical Drive, #05-COR, 117456, Singapore; Department of Biomedical Engineering, National University of Singapore, 21 Lower Kent Ridge Rd, 119077, Singapore
Yen-Yu Ian Shih: Department of Neurology, University of North Carolina at Chapel Hill, 170 Manning Drive, Chapel Hill, NC 27599, USA
Eddie Tung Wee Tan: Singapore Institute for Neurotechnology (SINAPSE), National University of Singapore, 28 Medical Drive, #05-COR, 117456, Singapore; Department of Neurosurgery, National Neuroscience Institute (NNI), 11 Jalan Tan Tock Seng, 308433, Singapore; SingHealth Duke-NUS Neuroscience Academic Clinical Program, National Neuroscience Institute (NNI), 11 Jalan Tan Tock Seng, 308433 Singapore
Wen Li: Department of Electrical and Computer Engineering, Michigan State University, 428 S Shaw Ln, RM 2120 Engineering Building, MI 48824, USA
Wai Hoe Ng: Department of Neurosurgery, National Neuroscience Institute (NNI), 11 Jalan Tan Tock Seng, 308433, Singapore; SingHealth Duke-NUS Neuroscience Academic Clinical Program, National Neuroscience Institute (NNI), 11 Jalan Tan Tock Seng, 308433 Singapore
Hsin-Yi Lai: Interdisciplinary Institute of Neuroscience and Technology, Qiushi Academy for Advanced Studies, Zhejiang University, No. 268, Kaixuan Road, Hangzhou, Zhejiang 310027, China
You-Yin Chen: Department of Biomedical Engineering, National Yang Ming University, No. 155, Sec. 2, Linong St., Taipei 112, Taiwan, ROC
Nitish V. Thakor: Singapore Institute for Neurotechnology (SINAPSE), National University of Singapore, 28 Medical Drive, #05-COR, 117456, Singapore; Department of Electrical and Computer Engineering, National University of Singapore, 21 Lower Kent Ridge Rd, 119077, Singapore; Department of Biomedical Engineering, National University of Singapore, 21 Lower Kent Ridge Rd, 119077, Singapore; Department of Biomedical Engineering, Johns Hopkins University, Traylor 701/720 Rutland Ave, Baltimore, MD 21205, USA

Journal volume & issue: Vol. 82
pp. 455 – 465

Abstract

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This study developed a novel system combining a 16-channel micro-electrocorticography (μECoG) electrode array and functional photoacoustic microscopy (fPAM) to examine changes in neurovascular functions following transient ischemic attack (TIA) in rats. To mimic the pathophysiology of TIA, a modified photothrombotic ischemic model was developed by using 3 min illumination of 5 mW continuous-wave (CW) green laser light focusing on a distal branch of the middle cerebral artery (MCA). Cerebral blood volume (CBV), hemoglobin oxygen saturation (SO2), somatosensory evoked potentials (SSEPs) and alpha-to-delta ratio (ADR) were measured pre- and post-ischemia over a focal cortical region (i.e., 1.5 × 1.5 mm2). Unexpectedly, the SO2, peak-to-peak amplitude (PPA) of SSEPs and ADR recovered and achieved levels greater than the baseline values at the 4th hour post-ischemia induction without any intervention, whereas the CBV value only partially recovered. In other words, transient ischemia led to increased neural activity when the relative CBV was reduced, which may further compromise neural integrity or lead to subsequent vascular disease. This novel μECoG-fPAM system complements currently available imaging techniques and represents a promising technology for studying neurovascular coupling in animal models.

Published in Neurobiology of Disease

ISSN: 0969-9961 (Print); 1095-953X (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry
Website: https://www.journals.elsevier.com/neurobiology-of-disease

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