Xylanase Production by a Local Isolate, Trichoderma spp. FETL c3-2 via Solid State Fermentation Using Agricultural Wastes as Substrates

Abstract

The production of xylanase by a local isolate Trichoderma spp. FETL c3-2 via solid state fermentation system using sugar cane baggase:palm kernel cake as substrates was investigated. The optimized solid state fermentation (SSF) system consists of 5 g of sugar cane baggase: palm kernel cake of ratio 90:10 (%, w/w), moisture content of 75% (v/w), pH of moistening agent of pH 7.0, at 30C and inoculum size of 1x10^8 spores/ml. The SSF system was also supplemented with 4% (w/w) dextrin and 6% (w/w) of tryptone as additional carbon and nitrogen sources, respectively. Cellulose at the concentration of 0.2% (w/w) was found to be a significant inducer for xylanase production. Using the optimized SSF system, a maximum xylanase productivity of 75.0 U per mg glucosamine after 4 days of fermentation time at 30C was obtained. The modifications of the SSF system resulted an increase in xylanase productivity by 180% and growth by 40% compared to the basal SSF system.

Keywords

• Xylanase production
• Solid state fermentation
• Trichoderma spp.
• Agricultural wastes