Monoclonal antibody against Rab8 from Bombyx mori (Lepidoptera: Bombycidae)

Tomohide UNO; Takuya NAKADA; Yuichi UNO; Kengo KANAMARU; Hiroshi YAMAGATA; Masahiko NAKAMURA; Michihiro TAKAGI

doi:10.14411/eje.2007.080

European Journal of Entomology (Oct 2007)

Monoclonal antibody against Rab8 from Bombyx mori (Lepidoptera: Bombycidae)

Tomohide UNO,
Takuya NAKADA,
Yuichi UNO,
Kengo KANAMARU,
Hiroshi YAMAGATA,
Masahiko NAKAMURA,
Michihiro TAKAGI

Affiliations

Tomohide UNO: Laboratory of Biological Chemistry, Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Nada-ku, Hyogo 657-8501, Japan; e-mail: [email protected]
Takuya NAKADA: Laboratory of Biological Chemistry, Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Nada-ku, Hyogo 657-8501, Japan; e-mail: [email protected]
Yuichi UNO: Department of Plant Resource Science, Faculty of Agriculture, Kobe University, Nada-ku, Hyogo 657-8501, Japan
Kengo KANAMARU: Laboratory of Biological Chemistry, Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Nada-ku, Hyogo 657-8501, Japan; e-mail: [email protected]
Hiroshi YAMAGATA: Laboratory of Biological Chemistry, Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Nada-ku, Hyogo 657-8501, Japan; e-mail: [email protected]
Masahiko NAKAMURA: Department of Bioscience and Biotechnology, Faculty of Bioenvironmental Science, Kyoto Gakuen University, 1-1 Nanjo, Sogabe, Kameoka, Kyoto 621-8555, Japan
Michihiro TAKAGI: Department of Animal Science, Faculty of Agriculture, Kobe University, Nada-ku, Hyogo 657-8501, Japan

DOI: https://doi.org/10.14411/eje.2007.080
Journal volume & issue: Vol. 104, no. 4
pp. 641 – 645

Abstract

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Small GTPases of the Rab family are key regulators of membrane trafficking. Monoclonal antibodies are useful tools for identifying proteins that interact with other proteins and for examining their tissue distribution. We selected a monoclonal antibody against Rab8 of Bombyx mori L. It specifically recognized amino acid residues 30-109, which are conserved among Rab8 proteins, and did not recognize any other Rab proteins. Western blotting using the antibody revealed one band in the brains of B. mori and rat. Far-Western blotting analysis detected three proteins interacting with Rab8. These results indicate that this antibody is useful for clarifying the physiological function of Rab8 of B. mori and other species. This is a report of a study on a monoclonal antibody against insect Rab protein.

Published in European Journal of Entomology

ISSN: 1210-5759 (Print); 1802-8829 (Online)
Publisher: Institute of Entomology, Biology Centre, Czech Academy of Science
Country of publisher: Czechia
LCC subjects: Science: Zoology
Website: https://www.eje.cz/

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