Near-infrared photobiomodulation of blood reversibly inhibits platelet reactivity and reduces hemolysis

Tomasz Walski; Karolina Grzeszczuk-Kuć; Katarzyna Gałecka; Natalia Trochanowska-Pauk; Raghvendra Bohara; Albert Czerski; Konstanty Szułdrzyński; Wiesław Królikowski; Jerzy Detyna; Małgorzata Komorowska

doi:10.1038/s41598-022-08053-y

Scientific Reports (Mar 2022)

Near-infrared photobiomodulation of blood reversibly inhibits platelet reactivity and reduces hemolysis

Tomasz Walski,
Karolina Grzeszczuk-Kuć,
Katarzyna Gałecka,
Natalia Trochanowska-Pauk,
Raghvendra Bohara,
Albert Czerski,
Konstanty Szułdrzyński,
Wiesław Królikowski,
Jerzy Detyna,
Małgorzata Komorowska

Affiliations

Tomasz Walski: Department of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Wrocław University of Science and Technology
Karolina Grzeszczuk-Kuć: Department of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Wrocław University of Science and Technology
Katarzyna Gałecka: Department of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Wrocław University of Science and Technology
Natalia Trochanowska-Pauk: Department of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Wrocław University of Science and Technology
Raghvendra Bohara: CÚRAM, SFI Research Centre for Medical Devices, National University of Ireland
Albert Czerski: Division of Pathophysiology, Department of Immunology, Pathophysiology and Veterinary Prevention, Wrocław University of Environmental and Life Sciences
Konstanty Szułdrzyński: Department of Anaesthesiology and Intensive Care, Central Clinical Hospital of the Ministry of Interior and Administration in Warsaw
Wiesław Królikowski: 2nd Department of Medicine, Intensive Care Unit, Medical College, Jagiellonian University
Jerzy Detyna: Department of Mechanics, Materials and Biomedical Engineering, Faculty of Mechanical Engineering, Wrocław University of Science and Technology
Małgorzata Komorowska: Department of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Wrocław University of Science and Technology

DOI: https://doi.org/10.1038/s41598-022-08053-y
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 12

Abstract

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Abstract Photobiomodulation (PBM) in the red/near-infrared (R/NIR) spectral range has become widely recognized due to its anti-inflammatory and cytoprotective potential. We aimed to assess the effects of blood PBM on platelets function and hemolysis in an in vitro setting. Porcine blood samples were separated into four aliquots for this study, one of which served as a control, while the other three were subjected to three different NIR PBM dosages. The platelet count and functions and the plasma free haemoglobin and osmotic fragility of red blood cells were measured during the experiment. The control group had a considerable drop in platelet number, but the NIR exposed samples had more minimal and strictly dose-dependent alterations. These modifications were consistent with ADP and collagen-induced platelet aggregation. Furthermore, red blood cells that had received PBM were more resistant to osmotic stress and less prone to hemolysis, as seen by a slightly lower quantity of plasma free hemoglobin. Here we showed under well-controlled in vitro conditions that PBM reversibly inhibits platelet activation in a dose-dependent manner and reduces hemolysis.

Published in Scientific Reports

ISSN: 2045-2322 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Medicine; Science
Website: https://www.nature.com/srep/

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