Highly Pathogenic H5 Influenza Viruses Isolated between 2016 and 2017 in Vietnamese Live Bird Markets

Lizheng Guan; Gongxun Zhong; Shufang Fan; Erin M. Plisch; Robert Presler; Chunyang Gu; Lavanya Babujee; David Pattinson; Hang Le Khanh Nguyen; Vu Mai Phuong Hoang; Mai Quynh Le; Harm van Bakel; Gabriele Neumann; Yoshihiro Kawaoka

doi:10.3390/v15051093

Viruses (Apr 2023)

Highly Pathogenic H5 Influenza Viruses Isolated between 2016 and 2017 in Vietnamese Live Bird Markets

Lizheng Guan,
Gongxun Zhong,
Shufang Fan,
Erin M. Plisch,
Robert Presler,
Chunyang Gu,
Lavanya Babujee,
David Pattinson,
Hang Le Khanh Nguyen,
Vu Mai Phuong Hoang,
Mai Quynh Le,
Harm van Bakel,
Gabriele Neumann,
Yoshihiro Kawaoka

Affiliations

Lizheng Guan: Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53711, USA
Gongxun Zhong: Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53711, USA
Shufang Fan: Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53711, USA
Erin M. Plisch: Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53711, USA
Robert Presler: Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53711, USA
Chunyang Gu: Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53711, USA
Lavanya Babujee: Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53711, USA
David Pattinson: Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53711, USA
Hang Le Khanh Nguyen: National Institute of Hygiene and Epidemiology, Hanoi 100000, Vietnam
Vu Mai Phuong Hoang: National Institute of Hygiene and Epidemiology, Hanoi 100000, Vietnam
Mai Quynh Le: National Institute of Hygiene and Epidemiology, Hanoi 100000, Vietnam
Harm van Bakel: Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA
Gabriele Neumann: Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53711, USA
Yoshihiro Kawaoka: Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53711, USA

DOI: https://doi.org/10.3390/v15051093
Journal volume & issue: Vol. 15, no. 5
p. 1093

Abstract

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Routine surveillance in live poultry markets in the northern regions of Vietnam from 2016 to 2017 resulted in the isolation of 27 highly pathogenic avian H5N1 and H5N6 viruses of 3 different clades (2.3.2.1c, 2.3.4.4f, and 2.3.4.4g). Sequence and phylogenetic analysis of these viruses revealed reassortment with various subtypes of low pathogenic avian influenza viruses. Deep-sequencing identified minor viral subpopulations encoding variants that may affect pathogenicity and sensitivity to antiviral drugs. Interestingly, mice infected with two different clade 2.3.2.1c viruses lost body weight rapidly and succumbed to virus infection, whereas mice infected with clade 2.3.4.4f or 2.3.4.4g viruses experienced non-lethal infections.

Published in Viruses

ISSN: 1999-4915 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.mdpi.com/journal/viruses

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