Spectrophotometric Determination of Ceftriaxone Using 4-Dimethylaminobenzaldehyde

Abstract

A new spectrophotometric method has been developed for the determination of the potent antibiotic ceftriaxone (CF) by derivatization with 4-dimethylaminobenzaldehyde (DAB). The derivative indicated molar absorptivity of 5.3 x 103 L mol-1 cm-1 at λmax 397 nm and obeyed the Beer’s law within 20-100 µgmL-1. The color reaction was highly stable and did not show any change in absorbance up to 24 h. The method was successfully applied for the determination of CF from various pharmaceutical preparations available in local market. The amounts of CF found in various pharmaceutical preparations were within 237.4-990 mg ampoule-1 with standard deviation (SD) ± 0.0004-0.044 (n=3) respectively.

Keywords

• Ceftriaxone
• 4-dimethylaminobenzaldehyde
• spectrophotometry.