In silico selection approach to develop DNA aptamers for a stem-like cell subpopulation of non-small lung cancer adenocarcinoma cell line A549

Vidic Mateja; Smuc Tina; Janez Nika; Blank Michael; Accetto Tomaz; Mavri Jan; Nascimento Isis C.; Nery Arthur A.; Ulrich Henning; Lah Tamara T.

doi:10.2478/raon-2018-0014

Radiology and Oncology (Mar 2018)

In silico selection approach to develop DNA aptamers for a stem-like cell subpopulation of non-small lung cancer adenocarcinoma cell line A549

Vidic Mateja,
Smuc Tina,
Janez Nika,
Blank Michael,
Accetto Tomaz,
Mavri Jan,
Nascimento Isis C.,
Nery Arthur A.,
Ulrich Henning,
Lah Tamara T.

Affiliations

Vidic Mateja: Department of Genetic Toxicology and Cancer Biology, National Institute of Biology, Ljubljana, Slovenia
Smuc Tina: Centre of Excellence for Biosensors, Instrumentation and Process Control, Centre for Biotechnology, Ajdovščina, Slovenia
Janez Nika: Centre of Excellence for Biosensors, Instrumentation and Process Control, Centre for Biotechnology, Ajdovščina, Slovenia
Blank Michael: AptaIT GmbH, Munich, Germany
Accetto Tomaz: Department of Animal sciences, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
Mavri Jan: Centre of Excellence for Biosensors, Instrumentation and Process Control, Centre for Biotechnology, Ajdovščina, Slovenia
Nascimento Isis C.: Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil
Nery Arthur A.: Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil
Ulrich Henning: Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil
Lah Tamara T.: Department of Genetic Toxicology and Cancer Biology, National Institute of Biology, Ljubljana, Slovenia

DOI: https://doi.org/10.2478/raon-2018-0014
Journal volume & issue: Vol. 52, no. 2
pp. 152 – 159

Abstract

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Detection of circulating lung cancer cells with cancer-stem like characteristics would represent an improved tool for disease prognosis. However, current antibodies based methods have some disadvantages and therefore cell SELEX (Systematic Evolution of Ligands by Exponential Enrichment) was used to develop DNA aptamers, recognizing cell surface markers of non-small lung carcinoma (NSLC) cells.

Published in Radiology and Oncology

ISSN: 1581-3207 (Online)
Publisher: Sciendo
Country of publisher: Slovenia
LCC subjects: Medicine: Medicine (General): Medical physics. Medical radiology. Nuclear medicine
Website: https://sciendo.com/journal/RAON

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