Development and Validation of HLPC Method for the Estimation of Lamotrigine in Bulk and Pharmaceutical Formulations

Abstract

A simple, precise, accurate, and rapid HPLC method was developed to estimate the amount of lamotrigine in bulk and its pharmaceutical formulations. Waters Alliance HPLC system equipped with autosampler, ultraviolet detector, and Symmetry C8 (4.6 mm ID × 150 mm, 3.5 μm, Make: XTerra) column were used for the quantification of the drug. Separation was carried out at a flow rate of 0.7 mL/min. of mobile phase (acetonitrile and potassium dihydrogen phosphate buffer of pH=7.0 in the ratio 60 : 40 v/v), and the detection was carried out at a wavelength of 215 nm. The retention time of lamotrigine was found to be 2.797 min. The linearity was obeyed in the range of concentration 5–25 μg/mL. The developed method was found to be repeatable and reproducible; hence, it can be used as an alternative method in assay of the lamotrigine in any pharmaceutical industries.