Response of Liver Tissue Bax and Bcl-2 Gene Expression to Aerobic Training with L-Carnitine Supplementation in Rats Toxicated by Boldenone

Abstract

Objective: This study aimed to compare the response of liver tissue BAX and BCL-2 gene expression to aerobic training with L-carnitine supplementation in rats intoxicated by Boldenone. Methods: In this experimental study, 30 male Wistar rats, aged 12 weeks (weight 195±7.94 g) were randomly divided into five groups: Control, no-treatment, boldenone (5 mg/kg), L-carnitine (100 mg/kg) and aerobic training- L-carnitine. The moderate endurance intensity training program (50%-55% of maximal oxygen consumption) performed for 6 weeks and 5 times a week. Injection once a week in the quadriceps and hamstring was conducted in-depth. After anesthesia, an autopsy was performed, and the liver Isolated. The hepatic apoptosis gene expression in the samples was measured by Real-Time PCR. Data were analyzed by 1-way ANOVA and post hoc Scheffe at the significant level P<0.05. Results: Significant difference was observed between the mean expression of BAX and BCL-2 in the liver tissue of male Wistar rats in different groups (P=0.001). The BAX gene expression of the liver tissue in L-carnitine -aerobic training and L-carnitine groups was significantly lower than the Boldenon group (P=0.001). Also, The BCL-2 gene expression in L-carnitine- aerobic exercise and L-carnitine groups was significantly higher than the Boldenon group (P=0.001). Conclusion: According to the findings of this study, supplementation of L-carnitine with regular aerobic training can have a protective effect against apoptosis induced by anabolic-androgenic steroids.

Keywords

• aerobic training
• boldenone
• l-carnitine
• apoptosis
• wistar rats