Journal of Cotton Research (Dec 2023)

Biocontrol potential of entomopathogenic nematode, Heterorhabditis indica against pink bollworm, Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae)

  • Shivaji Thube,
  • Swati Shinde,
  • Vivek Shah,
  • Nandini Gokte-Narkhedkar,
  • Dnyaneshwar Ingole,
  • Akash Nikoshe,
  • Prabhulinga Tenguri,
  • Snehal Thavkar,
  • Babasaheb Fand,
  • Vrushali Deshmukh,
  • Yenumula Prasad

DOI
https://doi.org/10.1186/s42397-023-00159-6
Journal volume & issue
Vol. 6, no. 1
pp. 1 – 11

Abstract

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Abstract Background The emergence of pink bollworm (PBW), Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae), in cotton due to Bt resistance and concealed feeding habit has created a need for alternative, eco-friendly, and cost-effective control methods. This study aimed to evaluate the bio-efficacy and reproductive potential of two native strains of entomopathogenic nematodes (EPNs), Heterorhabditis indica, namely CICR-HI-CL and CICR-HI-MN, against PBW larvae and pupae under in-vitro conditions. Results The larval assay revealed that strain CICR-HI-CL exhibited higher potency than strain CICR-HI-MN against 2nd, 3rd, and 4th instar larvae, with median lethal dose (LD 50) values of 5.45, 4.45, and 4.60 infective juveniles (IJs) per larva, respectively. In case of pupal bioassay, both EPN strains demonstrated greater virulence when applied directly (LD 50 values: 29.65 and 73.88 IJs per pupa for strains CICR-HI-CL and CICR-HI-MN, respectively) compared to soil application (147.84 and 272.38 IJs per pupa). Both EPN strains successfully penetrated and reproduced on 4th instar larvae, resulting in maximum production of 19.28 and 20.85 lakh IJs per larva in the next generation when inoculated at 30 IJs per larva. Conclusion The present study has generated useful information on the virulence and reproductive potential of two strains of EPN H. indica (CICR-HI-CL and CICR-HI-MN) against PBW, a dreaded pest of cotton. Higher virulence and reproductive potential of EPN strains demonstrated their ability to multiply, sustain and perpetuate on larval and pupal stages of PBW. The knowledge generated will help formulate effective management strategies for PBW with the inclusion of EPN as a potential biological control candidate. The soil-dwelling life stages viz., last instar hibernating larvae and pupae of PBW can be the ideal weak links to make a successful use of H. indica for sustainable management of PBW in the cotton ecosystem. However, before taking these EPN strains to field for managing PBW, detailed studies investigating their biocontrol potential against PBW under field conditions are needed.

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