Rapid Mass Spectrometric Study of a Supercritical CO₂-extract from Woody Liana <i>Schisandra chinensis</i> by HPLC-SPD-ESI-MS/MS

Abstract

Woody liana Schisandra chinensis contains valuable lignans, which are phenylpropanoids with valuable biological activity. Among green and selective extraction methods, supercritical carbon dioxide (SC-CO2) was shown to be the method of choice for the recovery of these naturally occurring compounds. Carbon dioxide (CO2) was the solvent with the flow rate (10−25 g/min) with 2% ethanol as co-solvent. In this piece of work operative parameters and working conditions were optimized by experimenting with different pressures (200–400 bars) and temperatures (40–60 °C). The extraction time varied from 60 to 120 min. HPLC-SPD-ESI -MS/MS techniques were applied to detect target analytes. Twenty-six different lignans were identified in the S. chinensis SC-CO2 extracts.

Keywords

• <i>Schisandra chinensis</i>
• supercritical fluid extraction
• HPLC-SPD-ESI-MS/MS
• lignans