DNA Vaccine Encoding a Modified Hemagglutinin Trimer of Avian Influenza A Virus H5N8 Protects Mice from Viral Challenge

Victoria R. Litvinova; Andrey P. Rudometov; Nadezhda B. Rudometova; Denis N. Kisakov; Mariya B. Borgoyakova; Lyubov A. Kisakova; Ekaterina V. Starostina; Anastasia A. Fando; Vladimir A. Yakovlev; Elena V. Tigeeva; Ksenia I. Ivanova; Andrei S. Gudymo; Tatiana N. Ilyicheva; Vasiliy Yu. Marchenko; Artemiy A. Sergeev; Alexander A. Ilyichev; Larisa I. Karpenko

doi:10.3390/vaccines12050538

Vaccines (May 2024)

DNA Vaccine Encoding a Modified Hemagglutinin Trimer of Avian Influenza A Virus H5N8 Protects Mice from Viral Challenge

Victoria R. Litvinova,
Andrey P. Rudometov,
Nadezhda B. Rudometova,
Denis N. Kisakov,
Mariya B. Borgoyakova,
Lyubov A. Kisakova,
Ekaterina V. Starostina,
Anastasia A. Fando,
Vladimir A. Yakovlev,
Elena V. Tigeeva,
Ksenia I. Ivanova,
Andrei S. Gudymo,
Tatiana N. Ilyicheva,
Vasiliy Yu. Marchenko,
Artemiy A. Sergeev,
Alexander A. Ilyichev,
Larisa I. Karpenko

Affiliations

Victoria R. Litvinova: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Andrey P. Rudometov: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Nadezhda B. Rudometova: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Denis N. Kisakov: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Mariya B. Borgoyakova: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Lyubov A. Kisakova: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Ekaterina V. Starostina: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Anastasia A. Fando: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Vladimir A. Yakovlev: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Elena V. Tigeeva: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Ksenia I. Ivanova: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Andrei S. Gudymo: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Tatiana N. Ilyicheva: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Vasiliy Yu. Marchenko: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Artemiy A. Sergeev: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Alexander A. Ilyichev: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Larisa I. Karpenko: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia

DOI: https://doi.org/10.3390/vaccines12050538
Journal volume & issue: Vol. 12, no. 5
p. 538

Abstract

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The development of a safe and effective vaccine against avian influenza A virus (AIV) H5N8 is relevant due to the widespread distribution of this virus in the bird population and the existing potential risk of human infection, which can lead to significant public health concerns. Here, we developed an experimental pVAX-H5 DNA vaccine encoding a modified trimer of AIV H5N8 hemagglutinin. Immunization of BALB/c mice with pVAX-H5 using jet injection elicited high titer antibody response (the average titer in ELISA was 1 × 105), and generated a high level of neutralizing antibodies against H5N8 and T-cell response, as determined by ELISpot analysis. Both liquid and lyophilized forms of pVAX-H5 DNA vaccine provided 100% protection of immunized mice against lethal challenge with influenza A virus A/turkey/Stavropol/320-01/2020 (H5N8). The results obtained indicate that pVAX-H5 has good opportunities as a vaccine candidate against the influenza A virus (H5N8).

Published in Vaccines

ISSN: 2076-393X (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine
Website: http://www.mdpi.com/journal/vaccines

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