Discovery of multiple anti-CRISPRs highlights anti-defense gene clustering in mobile genetic elements

Rafael Pinilla-Redondo; Saadlee Shehreen; Nicole D. Marino; Robert D. Fagerlund; Chris M. Brown; Søren J. Sørensen; Peter C. Fineran; Joseph Bondy-Denomy

doi:10.1038/s41467-020-19415-3

Nature Communications (Nov 2020)

Discovery of multiple anti-CRISPRs highlights anti-defense gene clustering in mobile genetic elements

Rafael Pinilla-Redondo,
Saadlee Shehreen,
Nicole D. Marino,
Robert D. Fagerlund,
Chris M. Brown,
Søren J. Sørensen,
Peter C. Fineran,
Joseph Bondy-Denomy

Affiliations

Rafael Pinilla-Redondo: Section of Microbiology, University of Copenhagen
Saadlee Shehreen: Department of Biochemistry, University of Otago
Nicole D. Marino: Department of Microbiology and Immunology, University of California
Robert D. Fagerlund: Department of Microbiology and Immunology, University of Otago
Chris M. Brown: Department of Biochemistry, University of Otago
Søren J. Sørensen: Section of Microbiology, University of Copenhagen
Peter C. Fineran: Department of Microbiology and Immunology, University of Otago
Joseph Bondy-Denomy: Department of Microbiology and Immunology, University of California

DOI: https://doi.org/10.1038/s41467-020-19415-3
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 11

Abstract

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Mobile genetic elements have evolved anti-CRISPR (Acr) proteins to bypass the immunity provided by prokaryotic CRISPR–Cas systems. Here, the authors identify 11 type I Acrs encoded on mobile genetic elements, and show that acr loci neighborhoods can be used to discover inhibitors of other bacterial defense systems.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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