Elaboration of test for enantiomeric purity determination of chiral monomers of polyamide mimetics of nucleic acids

Abstract

An approach for the determination of enantiomeric purity of chiral monomers of α-polyamide mimetics of nucleic acids is described. The test consisted of three steps: synthesis of each monomer as a racemic mixture from DL-Glu or DL-Ala, then selection of an eluent system for direct enantiomers separation on chiral phase by HPLC, and subsequent estimation of the amount of the major enantiomer obtained from L-Ala or L-Glu

Keywords

• peptide nucleic acids (pna)
• polyamide mimetics of nucleic acids
• enantiomeric purity
• chiral sorbent
• hplc