eLife (May 2015)

Quantitative isoform-profiling of highly diversified recognition molecules

  • Dietmar Schreiner,
  • Jovan Simicevic,
  • Erik Ahrné,
  • Alexander Schmidt,
  • Peter Scheiffele

DOI
https://doi.org/10.7554/eLife.07794
Journal volume & issue
Vol. 4

Abstract

Read online

Complex biological systems rely on cell surface cues that govern cellular self-recognition and selective interactions with appropriate partners. Molecular diversification of cell surface recognition molecules through DNA recombination and complex alternative splicing has emerged as an important principle for encoding such interactions. However, the lack of tools to specifically detect and quantify receptor protein isoforms is a major impediment to functional studies. We here developed a workflow for targeted mass spectrometry by selected reaction monitoring that permits quantitative assessment of highly diversified protein families. We apply this workflow to dissecting the molecular diversity of the neuronal neurexin receptors and uncover an alternative splicing-dependent recognition code for synaptic ligands.

Keywords