Quantitative semimicro analysis of triglyceride fatty acid distribution in a Congo palm oil

Abstract

The triglycerides of a Congo palm oil were separated into fractions with 0, 1, 2, 3, and 4 double bonds by thin-layer chromatography using silica gel impregnated with silver nitrate as adsorbent. The amounts and fatty acid compositions of these fractions were determined. In addition, the fatty acid compositions at the 2-positions of the palm oil and of the triglyceride fractions were determined by lipase splitting. The analytical results allow an accurate determination of the triglyceride composition. The results confirm the correctness of Vander Wal's theory on the distribution of fatty acids in triglycerides of natural fats.