Genome-wide identification and analysis of wheat LRR-RLK family genes following Chinese wheat mosaic virus infection

Shuang Liu; Shuang Liu; Shuang Liu; Jiajia Lei; Jiajia Lei; Juan Zhang; Juan Zhang; Hanhong Liu; Zhuangxin Ye; Zhuangxin Ye; Jin Yang; Jin Yang; Qiseng Lu; Qiseng Lu; Peng Liu; Peng Liu; Jianping Chen; Jianping Chen; Jian Yang; Jian Yang

doi:10.3389/fpls.2022.1109845

Frontiers in Plant Science (Jan 2023)

Genome-wide identification and analysis of wheat LRR-RLK family genes following Chinese wheat mosaic virus infection

Shuang Liu,
Shuang Liu,
Shuang Liu,
Jiajia Lei,
Jiajia Lei,
Juan Zhang,
Juan Zhang,
Hanhong Liu,
Zhuangxin Ye,
Zhuangxin Ye,
Jin Yang,
Jin Yang,
Qiseng Lu,
Qiseng Lu,
Peng Liu,
Peng Liu,
Jianping Chen,
Jianping Chen,
Jian Yang,
Jian Yang

Affiliations

Shuang Liu: College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China
Shuang Liu: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, China
Shuang Liu: Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China
Jiajia Lei: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, China
Jiajia Lei: Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China
Juan Zhang: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, China
Juan Zhang: Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China
Hanhong Liu: Junan County Bureau of Agriculture and Country, Linyi, China
Zhuangxin Ye: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, China
Zhuangxin Ye: Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China
Jin Yang: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, China
Jin Yang: Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China
Qiseng Lu: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, China
Qiseng Lu: Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China
Peng Liu: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, China
Peng Liu: Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China
Jianping Chen: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, China
Jianping Chen: Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China
Jian Yang: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, China
Jian Yang: Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China

DOI: https://doi.org/10.3389/fpls.2022.1109845
Journal volume & issue: Vol. 13

Abstract

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BackgroundAs the largest plant receptor-like protein kinase (RLK) superfamily, the leucine-rich repeat receptor-like kinases (LRR-RLKs) family are involved in plant growth, development, and stress responses. However, the functions of LRR-RLKs in wheat immunity remain unknown.ResultsIn the current study, 929 LRR-RLKs were identified in Triticum aestivum genome database using the BLAST and hidden Markov models (HMM) approach and divided into 14 clades. Chromosomal localization and synteny analysis revealed that TaLRR-RLKs were randomly distributed on all chromosomes with 921 collinear events. Through the cis-acting elements analysis, we observed that TaLRR-RLKs participated in hormone response, light response, development, metabolism, and response to environmental stress. The transcript level of 14 random selected TaLRR-RLKs from each subfamily was regulated by plant hormone treatment and Chinese wheat mosaic virus (CWMV) infection. The function of TaLRR-RLKs in wheat resistance to CWMV infection was further investigated by virus-induced gene silencing assay. Additionally, the accumulation of MeJA response genes, as well as CWMV RNA were not changed in the TaLRR-RLK silencing plants under MeJA treatment.ConclusionsOur results demonstrated that TaLRR-RLKs play an important role in wheat resistance to viral infection via hormone signals and lay the groundwork for the functional study of TaLRR-RLKs in wheat.

Published in Frontiers in Plant Science

ISSN: 1664-462X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Agriculture: Plant culture
Website: https://www.frontiersin.org/journals/plant-science

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