Crystals (Dec 2021)
Effect of Nutrient Solution Composition on Bio-Cemented Sand
Abstract
Microbial-induced carbonate precipitation is an environmentally friendly foundation treatment technology that effectively improves soil engineering performance. The various nutrient components of liquid curing compounds significantly influence the curing effect. On the basis of penetration, dry density, water absorption, and unconfined compressive strength tests, this study showed the effect of nutrient solution composition, including urea, calcium chloride, sodium bicarbonate, ammonium chloride, and nutrient broth, on the physicomechanical properties of bio-cemented sand. The morphological differences of calcium carbonate precipitates under nutrient solution composition were compared through scanning electron microscopy (SEM). Results showed that the curing effect of compound nutrient solution was improved compared with the basic nutrient solution (urea and calcium chloride). Among the individual components added, ammonium chloride had the most remarkable effect, followed by sodium bicarbonate and nutrient broth. Among the paired components added, sodium bicarbonate + ammonium chloride had the most significant effect, followed by sodium bicarbonate + nutrient broth and ammonium chloride + nutrient broth. The strength of bio-cemented sand cured with compound nutrient solution containing five components could reach 3.43 MPa, which was 1.92 times higher than the strength of the basic nutrient solution. As shown by the SEM image, the calcium carbonate precipitation in the solidified sand was distributed in the clearance of sand particles, effectively bonding the sand particles. The calcium carbonate obtained by the composition of the compound nutrient solution precipitated the sand particles, and some of the sand particles were wrapped. Moreover, the amount of precipitation was evidently greater than that of the basic nutrient solution. Compared with the basic nutrient solution, the compound nutrient solution effectively reduced the apparent porosity and average pore size of the sand. Thus, the curing effect of the compound nutrient solution was better than that of the basic nutrient solution.
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