Nature Communications (Jun 2017)

Single-mutation fitness landscapes for an enzyme on multiple substrates reveal specificity is globally encoded

  • Emily E. Wrenbeck,
  • Laura R. Azouz,
  • Timothy A. Whitehead

DOI
https://doi.org/10.1038/ncomms15695
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 10

Abstract

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Systematically understanding the sequence determinants to substrate specificity for enzymes has implications in areas from evolutionary biology to biocatalysis. Here, Whitehead and colleagues generate and analyse near-comprehensive single-mutation fitness landscapes for an amidase with three different substrates.