Nature Communications (Mar 2021)

Structural resolution of switchable states of a de novo peptide assembly

  • William M. Dawson,
  • Eric J. M. Lang,
  • Guto G. Rhys,
  • Kathryn L. Shelley,
  • Christopher Williams,
  • R. Leo Brady,
  • Matthew P. Crump,
  • Adrian J. Mulholland,
  • Derek N. Woolfson

DOI
https://doi.org/10.1038/s41467-021-21851-8
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 10

Abstract

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So far most of the de novo designed proteins are for single states only. Here, the authors present the de novo design and crystal structure determination of a coiled-coil peptide that assembles into multiple, distinct conformational states under the same conditions and further characterise its properties with biophysical experiments, NMR and MD simulations.