Generation and characterization of human-derived iPSC lines from two cousins with schizophrenia and bipolar disorder and their unaffected cousin

Peiyan Ni; Chuqing Zhou; Yajing Meng; Rui Xue; Xia Yang; Lei Li; Liansheng Zhao; Jinxue Wei; Rongjun Ni; Yingcheng Wang; Xiaohong Ma; Wanjun Guo; Qiang Wang; Tao Li

Stem Cell Research (Aug 2022)

Generation and characterization of human-derived iPSC lines from two cousins with schizophrenia and bipolar disorder and their unaffected cousin

Peiyan Ni,
Chuqing Zhou,
Yajing Meng,
Rui Xue,
Xia Yang,
Lei Li,
Liansheng Zhao,
Jinxue Wei,
Rongjun Ni,
Yingcheng Wang,
Xiaohong Ma,
Wanjun Guo,
Qiang Wang,
Tao Li

Affiliations

Peiyan Ni: The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China
Chuqing Zhou: The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China
Yajing Meng: The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China
Rui Xue: The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China
Xia Yang: The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China
Lei Li: The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China
Liansheng Zhao: The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China
Jinxue Wei: The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China
Rongjun Ni: The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China
Yingcheng Wang: The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China
Xiaohong Ma: The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China
Wanjun Guo: The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China
Qiang Wang: The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China
Tao Li: Department of Neurobiology, Affiliated Mental Health Center & Hangzhou Seventh People's Hospital, Zhejiang University School of Medicine, Hangzhou 310013, Zhejiang, China; NHC and CAMS Key Laboratory of Medical Neurobiology, MOE Frontier Science Center for Brain Science and Brain-machine Integration, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou, Zhejiang, China; Corresponding author at: Affiliated Mental Health Centre & Hangzhou Seventh People's Hospital, Zhejiang University School of Medicine, Hangzhou 310013, Zhejiang, China.

Journal volume & issue: Vol. 63
p. 102832

Abstract

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Schizophrenia (SCZ) and bipolar disorder (BD) are debilitating neurodevelopmental disorders with high heritability. In this study, peripheral blood mononuclear cells (PBMCs) were donated by three females. An adolescent female was clinically diagnosed as first-episode SCZ. One of her cousins was clinically diagnosed as BD and another one was unaffected control. Induced pluripotent stem cells (iPSCs) were established with reprograming factors Oct4, Sox2, Nanog, Lin28, c-myc, Klf4, and SV40LT. All lines presented normal karyotype and highly expressed pluripotency markers in vitro. All iPSCs were capable to differentiate into derivatives of three germ layers in vivo.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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