A growth selection system for the directed evolution of amine-forming or converting enzymes

Shuke Wu; Chao Xiang; Yi Zhou; Mohammad Saiful Hasan Khan; Weidong Liu; Christian G. Feiler; Ren Wei; Gert Weber; Matthias Höhne; Uwe T. Bornscheuer

doi:10.1038/s41467-022-35228-y

Nature Communications (Dec 2022)

A growth selection system for the directed evolution of amine-forming or converting enzymes

Shuke Wu,
Chao Xiang,
Yi Zhou,
Mohammad Saiful Hasan Khan,
Weidong Liu,
Christian G. Feiler,
Ren Wei,
Gert Weber,
Matthias Höhne,
Uwe T. Bornscheuer

Affiliations

Shuke Wu: Department of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, University of Greifswald
Chao Xiang: Department of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, University of Greifswald
Yi Zhou: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University
Mohammad Saiful Hasan Khan: Department of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, University of Greifswald
Weidong Liu: Department of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, University of Greifswald
Christian G. Feiler: Macromolecular Crystallography, Helmholtz-Zentrum Berlin für Materialien und Energie
Ren Wei: Department of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, University of Greifswald
Gert Weber: Macromolecular Crystallography, Helmholtz-Zentrum Berlin für Materialien und Energie
Matthias Höhne: Protein Biochemistry, Institute of Biochemistry, University of Greifswald
Uwe T. Bornscheuer: Department of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, University of Greifswald

DOI: https://doi.org/10.1038/s41467-022-35228-y
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 10

Abstract

Read online

Fast screening of enzymes is key for directed evolution of industrial biocatalysts. Here, the authors report a simple, high-throughput, and low-equipment-dependent growth selection system for engineering three enzymes for synthesis of chiral amines.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal