Frontiers in Genetics (Dec 2020)

Promoter PPSP1–5-BnPSP-1 From Ramie (Boehmeria nivea L. Gaud.) Can Drive Phloem-Specific GUS Expression in Arabidopsis thaliana

  • Yunhe Wang,
  • Yaning Bao,
  • Yaning Bao,
  • Yancheng Zheng,
  • Ping’an Guo,
  • Dingxiang Peng,
  • Bo Wang

DOI
https://doi.org/10.3389/fgene.2020.553265
Journal volume & issue
Vol. 11

Abstract

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Isolation of phloem-specific promoters is one of the basic conditions for improving the fiber development and resistance of ramie phloem using genetic engineering. In this study, we isolated a ramie endogenous promoter (named PPSP1-BnPSP-1) and analyzed the function of its truncated fragments in Arabidopsis. The results show that PPSP1-BnPSP-1 can drive the GUS reporter gene to be specifically expressed in the veins of Arabidopsis. After hormone and simulated drought treatment of the independent Arabidopsis lines carrying PPSP1-BnPSP-1 and its truncated fragments, only PPSP1–5-BnPSP-1 (−600 to −1 bp region of PPSP1-BnPSP-1) is stably expressed and exhibits phloem specificity. Our findings suggest that PPSP1–5-BnPSP-1 can be used as a phloem specific promoter for further research.

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