In crystallo observation of three metal ion promoted DNA polymerase misincorporation

Caleb Chang; Christie Lee Luo; Yang Gao

doi:10.1038/s41467-022-30005-3

Nature Communications (Apr 2022)

In crystallo observation of three metal ion promoted DNA polymerase misincorporation

Caleb Chang,
Christie Lee Luo,
Yang Gao

Affiliations

Caleb Chang: Department of Biosciences, Rice University
Christie Lee Luo: Department of Biosciences, Rice University
Yang Gao: Department of Biosciences, Rice University

DOI: https://doi.org/10.1038/s41467-022-30005-3
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 11

Abstract

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By observing DNA polymerase misincorporation with time-resolved crystallography, the authors visualize three-metal ion dependent polymerase catalysis and identify A-site metal-mediated primer alignment as a key step in nucleotide discrimination.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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