Genetic diversity analysis of fertility restorer and CMS lines of wheat (Triticum aestivum L.) by using SSR markers

Abstract

Genetic diversity of 92 fertility restorer and four CMS lines were analyzed using 38 polymorphic SSR markers. A total of 102 alleles were detected which are ranged from 2 to 4 with an average of 2.68 alleles per locus. Polymorphism information content (PIC) of SSR markers ranged from 0.22 to 0.81 with an average of 0.53 per locus. The cluster analysis based on 38 polymorphic markers produced seven main clusters with 0.36 to 0.97 similarity coefficient. The highest similarity was observed between the restorer lines 919R and 920R and three restorer lines 907R, 923R, and 922R were found to be most distinct from the rest. Analysis of molecular variance revealed within and among populations variance of 97 and 3 per cent, respectively. Cluster analysis revealed the genetically distinct restorer and CMS lines that would serve as diverse parents to be exploited in future breeding programme to broaden the genetic base of wheat cultivars.

Keywords

• genetic diversity
• restorer line
• cms line
• ssr markers
• triticum aestivum