Frontiers in Plant Science (Aug 2023)
A molecular framework for lc controlled locule development of the floral meristem in tomato
- Hengzuo Xiang,
- Hengzuo Xiang,
- Hengzuo Xiang,
- Hengzuo Xiang,
- Hengzuo Xiang,
- Sida Meng,
- Sida Meng,
- Sida Meng,
- Sida Meng,
- Sida Meng,
- Yunzhu Ye,
- Yunzhu Ye,
- Yunzhu Ye,
- Yunzhu Ye,
- Yunzhu Ye,
- Leilei Han,
- Leilei Han,
- Leilei Han,
- Leilei Han,
- Leilei Han,
- Yi He,
- Yi He,
- Yiqing Cui,
- Yiqing Cui,
- Changhua Tan,
- Changhua Tan,
- Changhua Tan,
- Changhua Tan,
- Changhua Tan,
- Jian Ma,
- Jian Ma,
- Jian Ma,
- Jian Ma,
- Jian Ma,
- Mingfang Qi,
- Mingfang Qi,
- Mingfang Qi,
- Mingfang Qi,
- Mingfang Qi,
- Tianlai Li,
- Tianlai Li,
- Tianlai Li,
- Tianlai Li,
- Tianlai Li
Affiliations
- Hengzuo Xiang
- College of Horticulture, Shenyang Agricultural University, Shenyang, China
- Hengzuo Xiang
- National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning), Shenyang, China
- Hengzuo Xiang
- Key Laboratory of Protected Horticulture (Shenyang Agricultural University), Ministry of Education, Shenyang, China
- Hengzuo Xiang
- Key Laboratory of Horticultural Equipment, Ministry of Agriculture and Rural, Affairs P. R. China, Shenyang, China
- Hengzuo Xiang
- Collaborative Innovation Center of Protected Vegetable Provincial Co-construction Surrounds Bohai Gulf Region, Shenyang, China
- Sida Meng
- College of Horticulture, Shenyang Agricultural University, Shenyang, China
- Sida Meng
- National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning), Shenyang, China
- Sida Meng
- Key Laboratory of Protected Horticulture (Shenyang Agricultural University), Ministry of Education, Shenyang, China
- Sida Meng
- Key Laboratory of Horticultural Equipment, Ministry of Agriculture and Rural, Affairs P. R. China, Shenyang, China
- Sida Meng
- Collaborative Innovation Center of Protected Vegetable Provincial Co-construction Surrounds Bohai Gulf Region, Shenyang, China
- Yunzhu Ye
- College of Horticulture, Shenyang Agricultural University, Shenyang, China
- Yunzhu Ye
- National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning), Shenyang, China
- Yunzhu Ye
- Key Laboratory of Protected Horticulture (Shenyang Agricultural University), Ministry of Education, Shenyang, China
- Yunzhu Ye
- Key Laboratory of Horticultural Equipment, Ministry of Agriculture and Rural, Affairs P. R. China, Shenyang, China
- Yunzhu Ye
- Collaborative Innovation Center of Protected Vegetable Provincial Co-construction Surrounds Bohai Gulf Region, Shenyang, China
- Leilei Han
- College of Horticulture, Shenyang Agricultural University, Shenyang, China
- Leilei Han
- National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning), Shenyang, China
- Leilei Han
- Key Laboratory of Protected Horticulture (Shenyang Agricultural University), Ministry of Education, Shenyang, China
- Leilei Han
- Key Laboratory of Horticultural Equipment, Ministry of Agriculture and Rural, Affairs P. R. China, Shenyang, China
- Leilei Han
- Collaborative Innovation Center of Protected Vegetable Provincial Co-construction Surrounds Bohai Gulf Region, Shenyang, China
- Yi He
- National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning), Shenyang, China
- Yi He
- Key Laboratory of Protected Horticulture (Shenyang Agricultural University), Ministry of Education, Shenyang, China
- Yiqing Cui
- National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning), Shenyang, China
- Yiqing Cui
- Key Laboratory of Protected Horticulture (Shenyang Agricultural University), Ministry of Education, Shenyang, China
- Changhua Tan
- College of Horticulture, Shenyang Agricultural University, Shenyang, China
- Changhua Tan
- National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning), Shenyang, China
- Changhua Tan
- Key Laboratory of Protected Horticulture (Shenyang Agricultural University), Ministry of Education, Shenyang, China
- Changhua Tan
- Key Laboratory of Horticultural Equipment, Ministry of Agriculture and Rural, Affairs P. R. China, Shenyang, China
- Changhua Tan
- Collaborative Innovation Center of Protected Vegetable Provincial Co-construction Surrounds Bohai Gulf Region, Shenyang, China
- Jian Ma
- College of Horticulture, Shenyang Agricultural University, Shenyang, China
- Jian Ma
- National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning), Shenyang, China
- Jian Ma
- Key Laboratory of Protected Horticulture (Shenyang Agricultural University), Ministry of Education, Shenyang, China
- Jian Ma
- Key Laboratory of Horticultural Equipment, Ministry of Agriculture and Rural, Affairs P. R. China, Shenyang, China
- Jian Ma
- Collaborative Innovation Center of Protected Vegetable Provincial Co-construction Surrounds Bohai Gulf Region, Shenyang, China
- Mingfang Qi
- College of Horticulture, Shenyang Agricultural University, Shenyang, China
- Mingfang Qi
- National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning), Shenyang, China
- Mingfang Qi
- Key Laboratory of Protected Horticulture (Shenyang Agricultural University), Ministry of Education, Shenyang, China
- Mingfang Qi
- Key Laboratory of Horticultural Equipment, Ministry of Agriculture and Rural, Affairs P. R. China, Shenyang, China
- Mingfang Qi
- Collaborative Innovation Center of Protected Vegetable Provincial Co-construction Surrounds Bohai Gulf Region, Shenyang, China
- Tianlai Li
- College of Horticulture, Shenyang Agricultural University, Shenyang, China
- Tianlai Li
- National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning), Shenyang, China
- Tianlai Li
- Key Laboratory of Protected Horticulture (Shenyang Agricultural University), Ministry of Education, Shenyang, China
- Tianlai Li
- Key Laboratory of Horticultural Equipment, Ministry of Agriculture and Rural, Affairs P. R. China, Shenyang, China
- Tianlai Li
- Collaborative Innovation Center of Protected Vegetable Provincial Co-construction Surrounds Bohai Gulf Region, Shenyang, China
- DOI
- https://doi.org/10.3389/fpls.2023.1249760
- Journal volume & issue
-
Vol. 14
Abstract
Malformed tomato fruit with multiple locules is a common physiological disorder that significantly affects the quality of tomatoes. Research has shown that the occurrence of malformed fruit in tomatoes is closely linked to the number of locules, and two key QTLs, lc and fas, are involved in controlling this trait. It has been observed that lc has a relatively weaker effect on increasing locule number, which is associated with two SNPs in the CArG repressor element downstream of the SlWUS. However, the precise molecular mechanism underlying lc is not yet fully understood. In this study, we investigated the role of lc in tomato locule development. We found that the number of floral organs and fruit locules significantly increased in tomato lc knockout mutants. Additionally, these mutants showed higher expression levels of the SlWUS during carpel formation. Through cDNA library construction and yeast one-hybrid screening, we identified the MADS-box transcription factor SlSEP3, which was found to bind to lc. Furthermore, we observed an increase in floral organs and fruit locules similar to the lcCR plant on SlSEP3 silencing plants. However, it should be noted that the lc site is located after the 3′ untranslated region (UTR) of SlWUS in the tomato genome. As a result, SlSEP3 may not be able to exert regulatory functions on the promoter of the gene like other transcription factors. In the yeast two-hybrid assay, we found that several histone deacetylases (SlHDA1, SlHDA3, SlHDA4, SlHDA5, SlHDA6, SlHDA7, and SlHDA8) can interact with SlSEP3. This indicated that SlSEP3 can recruit these proteins to repress nucleosome relaxation, thereby inhibiting SlWUS transcription and affecting the number of locules in tomato fruit. Therefore, our findings reveal a new mechanism for lc playing a significant role in the genetic pathway regulating tomato locule development.
Keywords