International Journal of Mycobacteriology (Jan 2013)

Efficient discrimination by MIRU-VNTRs of Mycobacterium tuberculosis clinical isolates belonging to the predominant SIT11/EAI3-IND ancestral genotypic lineage in Kerala, India

  • Biljo V Joseph,
  • Smitha Soman,
  • Véronique Hill,
  • R Ajay Kumar,
  • Nalin Rastogi,
  • Sathish Mundayoor

DOI
https://doi.org/10.1016/j.ijmyco.2013.10.005
Journal volume & issue
Vol. 2, no. 4
pp. 244 – 247

Abstract

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The present study evaluated the ability of MIRU–VNTRs to discriminate Mycobacterium tuberculosis (MTB) clinical isolates belonging to the SIT11/EAI3-IND ancestral genotypic lineage, which is highly prevalent in Kerala, India. Starting from 168 MTB clinical isolates, spoligotyping (discriminatory index of 0.9113) differentiated the strains into 68 distinct patterns, the biggest cluster being SIT11/48 SIT11 (n = 48). The present study shows that 12-loci MIRUs and 3 ETRs allowed an efficient discrimination of these isolates (discriminatory indexes of 0.7819 and 0.5523, respectively).

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