Chemogenetic activation of nigrostriatal dopamine neurons in freely moving common marmosets

Koki Mimura; Yuji Nagai; Ken-ichi Inoue; Jumpei Matsumoto; Yukiko Hori; Chika Sato; Kei Kimura; Takashi Okauchi; Toshiyuki Hirabayashi; Hisao Nishijo; Noriaki Yahata; Masahiko Takada; Tetsuya Suhara; Makoto Higuchi; Takafumi Minamimoto

iScience (Sep 2021)

Chemogenetic activation of nigrostriatal dopamine neurons in freely moving common marmosets

Koki Mimura,
Yuji Nagai,
Ken-ichi Inoue,
Jumpei Matsumoto,
Yukiko Hori,
Chika Sato,
Kei Kimura,
Takashi Okauchi,
Toshiyuki Hirabayashi,
Hisao Nishijo,
Noriaki Yahata,
Masahiko Takada,
Tetsuya Suhara,
Makoto Higuchi,
Takafumi Minamimoto

Affiliations

Koki Mimura: Department of Functional Brain Imaging, National Institutes for Quantum and Radiological Science and Technology, Chiba 263-8555 Japan
Yuji Nagai: Department of Functional Brain Imaging, National Institutes for Quantum and Radiological Science and Technology, Chiba 263-8555 Japan
Ken-ichi Inoue: Systems Neuroscience Section, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan
Jumpei Matsumoto: Department of System Emotional Science, Faculty of Medicine, University of Toyama, Toyama 930-8555, Japan; Research Center for Idling Brain Science, University of Toyama, Toyama 930-8555, Japan
Yukiko Hori: Department of Functional Brain Imaging, National Institutes for Quantum and Radiological Science and Technology, Chiba 263-8555 Japan
Chika Sato: Quantum Life Informatics Group, Institute for Quantum Life Science, National Institutes for Quantum and Radiological Science and Technology, Chiba 263-8555 Japan; Applied MRI Research, Department of Molecular Imaging and Theranostics, National Institutes for Quantum and Radiological Science and Technology, Chiba 263-8555 Japan
Kei Kimura: Systems Neuroscience Section, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan
Takashi Okauchi: Department of Functional Brain Imaging, National Institutes for Quantum and Radiological Science and Technology, Chiba 263-8555 Japan
Toshiyuki Hirabayashi: Department of Functional Brain Imaging, National Institutes for Quantum and Radiological Science and Technology, Chiba 263-8555 Japan
Hisao Nishijo: Department of System Emotional Science, Faculty of Medicine, University of Toyama, Toyama 930-8555, Japan; Research Center for Idling Brain Science, University of Toyama, Toyama 930-8555, Japan
Noriaki Yahata: Quantum Life Informatics Group, Institute for Quantum Life Science, National Institutes for Quantum and Radiological Science and Technology, Chiba 263-8555 Japan; Applied MRI Research, Department of Molecular Imaging and Theranostics, National Institutes for Quantum and Radiological Science and Technology, Chiba 263-8555 Japan
Masahiko Takada: Systems Neuroscience Section, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan
Tetsuya Suhara: Department of Functional Brain Imaging, National Institutes for Quantum and Radiological Science and Technology, Chiba 263-8555 Japan
Makoto Higuchi: Department of Functional Brain Imaging, National Institutes for Quantum and Radiological Science and Technology, Chiba 263-8555 Japan
Takafumi Minamimoto: Department of Functional Brain Imaging, National Institutes for Quantum and Radiological Science and Technology, Chiba 263-8555 Japan; Corresponding author

Journal volume & issue: Vol. 24, no. 9
p. 103066

Abstract

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Summary: To interrogate particular neuronal pathways in nonhuman primates under natural and stress-free conditions, we applied designer receptors exclusively activated by designer drugs (DREADDs) technology to common marmosets. We injected adeno-associated virus vectors expressing the excitatory DREADD hM3Dq into the unilateral substantia nigra (SN) in four marmosets. Using multi-tracer positron emission tomography imaging, we detected DREADD expression in vivo, which was confirmed in nigrostriatal dopamine neurons by immunohistochemistry, as well as by assessed activation of the SN following agonist administration. The marmosets rotated in a contralateral direction relative to the activated side 30–90 min after consuming food containing the highly potent DREADD agonist deschloroclozapine (DCZ) but not on the following days without DCZ. These results indicate that non-invasive and reversible DREADD manipulation will extend the utility of marmosets as a primate model for linking neuronal activity and natural behavior in various contexts.

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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