Biosensors (Nov 2022)

The Simultaneous Determination of Chlorpyrifos–Ethyl and –Methyl with a New Format of Fluorescence-Based Immunochromatographic Assay

  • Zi-Hong Xu,
  • Jia Liu,
  • Bin Li,
  • Jun-Kai Wang,
  • Xi Zeng,
  • Zi-Jian Chen,
  • Surat Hongsibsong,
  • Wei Huang,
  • Hong-Tao Lei,
  • Yuan-Ming Sun,
  • Zhen-Lin Xu

DOI
https://doi.org/10.3390/bios12111006
Journal volume & issue
Vol. 12, no. 11
p. 1006

Abstract

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The improper and excessive use in agriculture of chlorpyrifos–methyl (CPSM) and chlorpyrifos–ethyl (CPSE) may affect the health of human beings. Herein, a fluorescence-based immunochromatographic assay (FICA) was developed for the simultaneous determination of CPSM and CPSE. A monoclonal antibody (mAb) with equal recognition of CPSM and CPSE was generated by the careful designing of haptens and screening of hybridoma cells. Instead of labeling fluorescence with mAb, the probe was labeled with goat-anti-mouse IgG (GAM-IgG) and pre-incubated with mAb in the sample. The complex could compete with CPS by coating antigen in the test line. The new format of FICA used goat-anti-rabbit IgG (GAR-IgG) conjugated with rabbit IgG labeled with fluorescence microspheres as an independent quality control line (C line). The novel strategy significantly reduced nonspecific reactions and increased assay sensitivity. Under the optimal conditions, the proposed FICA showed a linear range of 0.015–64 mg/L and limit of detection (LOD) of 0.015 mg/L for both CPSE and CPSM. The average recoveries of CPS from spiked food samples by FICA were 82.0–110.0%. The accuracy was similar to the gas chromatography–tandem mass spectrometry (GC-MS/MS) results. The developed FICA was an ideal on-site tool for rapid screening of CPS residues in foods.

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