The deubiquitinase USP21 maintains the stemness of mouse embryonic stem cells via stabilization of Nanog

Jiali Jin; Jian Liu; Cong Chen; Zhenping Liu; Cong Jiang; Hongshang Chu; Weijuan Pan; Xinbo Wang; Lingqiang Zhang; Bin Li; Cizhong Jiang; Xin Ge; Xin Xie; Ping Wang

doi:10.1038/ncomms13594

Nature Communications (Nov 2016)

The deubiquitinase USP21 maintains the stemness of mouse embryonic stem cells via stabilization of Nanog

Jiali Jin,
Jian Liu,
Cong Chen,
Zhenping Liu,
Cong Jiang,
Hongshang Chu,
Weijuan Pan,
Xinbo Wang,
Lingqiang Zhang,
Bin Li,
Cizhong Jiang,
Xin Ge,
Xin Xie,
Ping Wang

Affiliations

Jiali Jin: Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
Jian Liu: Chinese Academy of Sciences Key Laboratory of Receptor Research, National Center for Drug Screening, Shanghai Institute of Materia Medica, Chinese Academy of Sciences
Cong Chen: Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
Zhenping Liu: Department of Central Laboratory, Shanghai Tenth People’s Hospital of Tongji University, School of Life Science and Technology, Tongji University
Cong Jiang: Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
Hongshang Chu: Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
Weijuan Pan: Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
Xinbo Wang: Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
Lingqiang Zhang: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Collaborative Innovation Center for Cancer Medicine
Bin Li: Key Laboratory of Molecular Virology and Immunology, Unit of Molecular Immunology, Institute Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Cizhong Jiang: Department of Central Laboratory, Shanghai Tenth People’s Hospital of Tongji University, School of Life Science and Technology, Tongji University
Xin Ge: Department of Clinical Medicine, Shanghai Tenth People's Hospital of Tongji University, Tongji University
Xin Xie: Chinese Academy of Sciences Key Laboratory of Receptor Research, National Center for Drug Screening, Shanghai Institute of Materia Medica, Chinese Academy of Sciences
Ping Wang: Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University

DOI: https://doi.org/10.1038/ncomms13594
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 15

Abstract

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Nanog regulates embryonic stem cell (ESC) pluripotency but what controls Nanog protein stability is unclear. Here, the authors show that in mouse ESCs, Nanog protein is ubiquitinated and stabilized by the deubiquitinase USP21, which in turn is regulated by extrinsic signals, STAT3 and ERK.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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