Label-free DNA detection by loop- mediated isothermal amplification coupled with quartz crystal microbalance sensor

Abstract

Loop-mediated isothermal amplification (LAMP) and quartz crystal microbalance (QCM) sensors were used for DNA detection. Porcine DNA as DNA template was amplified and QCM sensors were then used to measure the changes in frequency in positive and negative samples. Mathematical models were employed to extract features from the sensor recordings by fitting the frequency -time plots using polynomial functions. The polynomial function coefficients of the plots gave valuable information for sample classification. Radar graphs and principle component analysis (PCA) were also applied to samples using these coefficients. Both the radar graphs and the PCA indicated differences between the sample groups when using coefficients of high-order functions.

Keywords

• label -free DNA detection
• DNA amplification
• quartz crystal microbalance
• loop-mediated isothermal amplification
• DNA