Human CKAP2L shows a cell cycle‐dependent expression pattern and exhibits microtubule‐stabilizing properties

Abstract

Cytoskeleton‐associated protein 2‐like (CKAP2L) is a paralogue of cytoskeleton‐associated protein 2 (CKAP2). We characterized the expression pattern, subcellular localization, and microtubule‐stabilizing properties of human CKAP2L. The levels of both CKAP2L transcript and protein were cell cycle phase‐dependent, peaking during the G2/M phase and relatively high in certain human tissues, including testis, intestine, and spleen. CKAP2L protein was detectable in all human cancer cell lines we tested. CKAP2L localized to the mitotic spindle apparatus during mitosis, as reported previously. During interphase, however, CKAP2L localized mainly to the nucleus. Ectopic overexpression of CKAP2L resulted in ‘microtubule bundling’, and, consequently, an elevated CKAP2L level led to prolonged mitosis. These findings support the mitotic role of CKAP2L during the human cell cycle.

Keywords

• cell cycle
• cytoskeleton‐associated protein 2
• cytoskeleton‐associated protein 2‐like
• microtubule
• mitosis
• mitotic spindle