Frontiers in Cellular and Infection Microbiology (Jun 2017)
p21-Activated Kinase 4 Signaling Promotes Japanese Encephalitis Virus-Mediated Inflammation in Astrocytes
- Wen He,
- Wen He,
- Wen He,
- Zikai Zhao,
- Zikai Zhao,
- Zikai Zhao,
- Awais Anees,
- Awais Anees,
- Awais Anees,
- Yunchuan Li,
- Yunchuan Li,
- Yunchuan Li,
- Usama Ashraf,
- Usama Ashraf,
- Usama Ashraf,
- Zheng Chen,
- Zheng Chen,
- Zheng Chen,
- Yunfeng Song,
- Yunfeng Song,
- Yunfeng Song,
- Huanchun Chen,
- Huanchun Chen,
- Huanchun Chen,
- Shengbo Cao,
- Shengbo Cao,
- Shengbo Cao,
- Jing Ye,
- Jing Ye,
- Jing Ye,
- Jing Ye
Affiliations
- Wen He
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural UniversityWuhan, China
- Wen He
- Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, China
- Wen He
- The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural UniversityWuhan, China
- Zikai Zhao
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural UniversityWuhan, China
- Zikai Zhao
- Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, China
- Zikai Zhao
- The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural UniversityWuhan, China
- Awais Anees
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural UniversityWuhan, China
- Awais Anees
- Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, China
- Awais Anees
- The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural UniversityWuhan, China
- Yunchuan Li
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural UniversityWuhan, China
- Yunchuan Li
- Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, China
- Yunchuan Li
- The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural UniversityWuhan, China
- Usama Ashraf
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural UniversityWuhan, China
- Usama Ashraf
- Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, China
- Usama Ashraf
- The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural UniversityWuhan, China
- Zheng Chen
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural UniversityWuhan, China
- Zheng Chen
- Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, China
- Zheng Chen
- The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural UniversityWuhan, China
- Yunfeng Song
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural UniversityWuhan, China
- Yunfeng Song
- Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, China
- Yunfeng Song
- The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural UniversityWuhan, China
- Huanchun Chen
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural UniversityWuhan, China
- Huanchun Chen
- Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, China
- Huanchun Chen
- The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural UniversityWuhan, China
- Shengbo Cao
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural UniversityWuhan, China
- Shengbo Cao
- Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, China
- Shengbo Cao
- The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural UniversityWuhan, China
- Jing Ye
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural UniversityWuhan, China
- Jing Ye
- Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, China
- Jing Ye
- The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural UniversityWuhan, China
- Jing Ye
- College of Life Science and Technology, Huazhong Agricultural UniversityWuhan, China
- DOI
- https://doi.org/10.3389/fcimb.2017.00271
- Journal volume & issue
-
Vol. 7
Abstract
Japanese encephalitis virus (JEV) targets central nervous system, resulting in neuroinflammation with typical features of neuronal death along with hyper activation of glial cells. Exploring the mechanisms responsible for the JEV-caused inflammatory response remains a pivotal area of research. In the present study, we have explored the function of p21-activated kinase 4 (PAK4) in JEV-mediated inflammatory response in human astrocytes. The results showed that JEV infection enhances the phosphorylation of PAK4 in U251 cells and mouse brain. Knockdown of PAK4 resulted in decreased expression of inflammatory cytokines that include tumor necrosis factor alpha, interleukin-6, interleukin-1β, and chemokine (C-C motif) ligand 5 and interferon β upon JEV infection, suggesting that PAK4 signaling promotes JEV-mediated inflammation. In addition, we found that knockdown of PAK4 led to the inhibition of MAPK signaling including ERK, p38 MAPK and JNK, and also resulted in the reduced nuclear translocation of NF-κB and phosphorylation of AP-1. These results demonstrate that PAK4 signaling actively promotes JEV-mediated inflammation in human astrocytes via MAPK-NF-κB/AP-1 pathway, which will provide a new insight into the molecular mechanism of the JEV-induced inflammatory response.
Keywords
